Effect of gallopamil on energy metabolism of the isolated perfused rat brain in the postischemic period.

The isolated perfused rat brain was used to demonstrate an effect of gallopamil on energy metabolism affected by ischemia. After a perfusion period of 30 min and 10 min of ischemia the isolated brain preparation was reperfused. From the onset of perfusion onwards, gallopamil (1 or 10 mumol/l) was present in the medium. The higher concentration of gallopamil accelerated significantly the restoration of the high-energy phosphates in the recovery stage: after 2 min of recirculation the ATP and the creatine-P levels were higher and the AMP level was lower in cortical tissue of drug-treated brains than in untreated controls. These results suggest that gallopamil protected brain energy metabolism against ischemic damage.