Li Xiaolu, Liu Dong, Han Bangxing, Huang Shiping, Deng Hui
College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
Anhui Province Key Laboratory of Conservation and Utilization for Dabie Mountain Special Bio-Resources, College of Biological and Pharmaceutical Engineering, West Anhui University, Luan, 237012, China.
Arch Microbiol. 2025 May 8;207(6):137. doi: 10.1007/s00203-025-04344-w.
The biosynthesis mechanism of ganoderic acid, a class of secondary metabolites of Ganoderma lucidum, is currently unknown. Members of G. lucidum's CYP450 gene family were found in this study by genome-wide analysis. Determination of ganoderic acid content in Jilin, Zhejiang, Jinzhai, Fujian, Yunnan and Shandong regions was performed using high performance liquid chromatography. Expression of CYP450 gene of G. lucidum from Jilin, Jinzhai and Shandong origins was analyzed by transcriptome sequencing. The findings indicated that, through comprehensive sequence analysis and functional annotation, 189 CYP450 genes were detected in the G. lucidum genome, among which 34 CYP450 genes were significantly differentially expressed in transcriptome analysis, and the gene expression was positively correlated with ganoderic acid content. Bioinformatics analysis predicted the conserved motifs, structural features, and subcellular localization of 189 CYP genes, revealing significant differences in gene structure and protein motif composition among GlCYP450 family members. Subcellular localization revealed that they are located in the plasma membrane, cytoplasm, nucleus, mitochondria, peroxisome, endoplasmic reticulum, extracellular space, and cytoskeleton, suggesting that they have multiple functions. A number of cis-regulatory elements associated with stress responses and phytohormones were identified in the promoter regions of these genes. It demonstrates that ganoderic acid production has been significantly controlled by these P450 genes. These findings offer a crucial theoretical foundation for a thorough comprehension of the process of ganoderic acid production, the bioinformatics role of CYP450 genes in G. lucidum, and the selection and breeding of superior G. lucidum resources.
灵芝酸是灵芝的一类次生代谢产物，其生物合成机制目前尚不清楚。本研究通过全基因组分析发现了灵芝CYP450基因家族的成员。采用高效液相色谱法测定了吉林、浙江、金寨、福建、云南和山东地区的灵芝酸含量。通过转录组测序分析了吉林、金寨和山东产地灵芝CYP450基因的表达情况。研究结果表明，通过综合序列分析和功能注释，在灵芝基因组中检测到189个CYP450基因，其中34个CYP450基因在转录组分析中显著差异表达，且基因表达与灵芝酸含量呈正相关。生物信息学分析预测了189个CYP基因的保守基序、结构特征和亚细胞定位，揭示了GlCYP450家族成员在基因结构和蛋白质基序组成上存在显著差异。亚细胞定位显示它们位于质膜、细胞质、细胞核、线粒体、过氧化物酶体、内质网、细胞外空间和细胞骨架中，表明它们具有多种功能。在这些基因的启动子区域鉴定出了一些与应激反应和植物激素相关的顺式调控元件。这表明灵芝酸的产生受到这些P450基因的显著控制。这些发现为深入了解灵芝酸的产生过程、CYP450基因在灵芝中的生物信息学作用以及优良灵芝资源的选育提供了重要的理论基础。
