Wu Huichao, Liu Jian, Jing Zhongjun, Qi Zihua, Ma Xianjun, Zhao Junying, Huang Laichong
Department of Emergency, The First People's Hospital of Jiashan, Jiashan, 314100, Zhejiang, China.
Department of Traditional Chinese Medicine for Pulmonology, China-Japan Friendship Hospital, Beijing, 100029, China.
J Cardiothorac Surg. 2025 May 9;20(1):228. doi: 10.1186/s13019-025-03438-2.
Early screening for lung cancer and early treatment of malignant pulmonary nodules (PNs) contribute a lot to reducing lung cancer mortality. Whether the highly expressed lncRNA KIF9-AS1 in multiple malignancies is associated with the development of malignant PNs is not clear. This study focused on the diagnosis and prognosis value of KIF9-AS1 in PNs patients.
The study population included 101 individuals with benign PNs and 172 patients diagnosed with malignant PNs. The expression of KIF9-AS1 was analyzed by qRT-PCR. The receiver operator characteristic (ROC) curve was plotted to evaluate the diagnostic performance while the Kaplan-Meier curve and multivariate Cox regression analysis were employed for prognosis. Correlation assessment was accomplished by Pearson correlation analysis.
Differentiated expression of KIF9-AS1 was found in benign and malignant PNs groups. The expression of KIF9-AS1 was positively associated with carcinoembryonic antigen (CEA) and cytokeratin 19 fragments 21 - 1 (CYFRA21-1). KIF9-AS1 presented high accuracy in distinguishing malignant PNs from benign PNs, especially combined with computed tomography (CT). The expression level of KIF9-AS1, and nodule diameter were independent risk factors of poor prognosis.
Assessment of serum KIF9-AS1 level contributed to clinical diagnosis and prognosis of malignant PNs and also improved the diagnostic value of CT.
肺癌的早期筛查以及恶性肺结节（PNs）的早期治疗对降低肺癌死亡率有很大帮助。多种恶性肿瘤中高表达的lncRNA KIF9-AS1是否与恶性PNs的发生有关尚不清楚。本研究聚焦于KIF9-AS1在PNs患者中的诊断及预后价值。
研究人群包括101例良性PNs患者和172例经诊断为恶性PNs的患者。采用qRT-PCR分析KIF9-AS1的表达。绘制受试者工作特征（ROC）曲线以评估诊断性能，同时采用Kaplan-Meier曲线和多因素Cox回归分析进行预后评估。通过Pearson相关分析进行相关性评估。
在良性和恶性PNs组中发现KIF9-AS1存在差异表达。KIF9-AS1的表达与癌胚抗原（CEA）和细胞角蛋白19片段21-1（CYFRA21-1）呈正相关。KIF9-AS1在区分恶性PNs和良性PNs方面具有较高的准确性，尤其是与计算机断层扫描（CT）联合使用时。KIF9-AS1的表达水平和结节直径是预后不良的独立危险因素。
血清KIF9-AS1水平的评估有助于恶性PNs的临床诊断和预后判断，同时提高了CT的诊断价值。
