Department of Cardiothoracic Surgery, Xuzhou No.1 People's Hospital, Xuzhou Municipal Hospital Affiliated with Xuzhou Medical College, 269 Daxue Road, Xuzhou, 221000, China.
Department of Respiratory Medicine, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China.
BMC Cancer. 2023 Aug 15;23(1):755. doi: 10.1186/s12885-023-11264-9.
This project aimed to research the significance of THRIL in the diagnosis of benign and malignant solitary pulmonary nodules (SPNs) and to investigate the role of THRIL/miR-99a in malignant SPNs.
The study groups consisted of 169 patients with SPN and 74 healthy subjects. The differences in THRIL levels were compared between the two groups and the healthy group. The receiver operating characteristic curve (ROC) was utilized to analyze the THRIL's significance in detecting benign and malignant SPN. Pearson correlation and binary regression coefficients represented the association between THRIL and SPN. CCK-8 assay, Transwell assay, and flow cytometry were utilized to detect the regulatory effect of THRIL silencing. The interaction between THRIL, miR-99a, and IGF1R was confirmed by the double luciferase reporter gene.
There were differences in THRIL expression in the healthy group, benign SPN group, and malignant SPN group. High accuracy of THRIL in the diagnosis of benign SPN and malignant SPN was observed. THRIL was associated with the development of SPN. The expression of THRIL was upregulated and miR-99a was downregulated in lung cancer cells. The double luciferase report experiment confirmed the connections between THRIL/miR-99a/IGF1R. Silencing THRIL could suppress cell proliferation, migration, and invasion and promote cell apoptosis by binding miR-99a.
The detection of THRIL in serum is useful for the assessment of malignant SPN. THRIL can regulate the expression of IGF1R through miR-99a, thereby promoting the growth of lung cancer cells and inhibiting apoptosis.
本项目旨在研究 THRIL 在良性和恶性孤立性肺结节（SPN）诊断中的意义，并探讨 THRIL/miR-99a 在恶性 SPN 中的作用。
研究组包括 169 例 SPN 患者和 74 例健康受试者。比较两组和健康组之间 THRIL 水平的差异。利用受试者工作特征曲线（ROC）分析 THRIL 在检测良性和恶性 SPN 中的意义。Pearson 相关系数和二元回归系数表示 THRIL 与 SPN 的相关性。CCK-8 检测、Transwell 检测和流式细胞术检测 THRIL 沉默的调节作用。双荧光素酶报告基因证实了 THRIL、miR-99a 和 IGF1R 之间的相互作用。
健康组、良性 SPN 组和恶性 SPN 组 THRIL 表达存在差异。THRIL 在诊断良性 SPN 和恶性 SPN 中具有较高的准确性。THRIL 与 SPN 的发生发展有关。肺癌细胞中 THRIL 表达上调，miR-99a 表达下调。双荧光素酶报告实验证实了 THRIL/miR-99a/IGF1R 之间的联系。沉默 THRIL 可以通过结合 miR-99a 抑制细胞增殖、迁移和侵袭，促进细胞凋亡。
血清中 THRIL 的检测有助于评估恶性 SPN。THRIL 可以通过 miR-99a 调节 IGF1R 的表达，从而促进肺癌细胞的生长并抑制凋亡。
