Li Haoran, Li Mingru, Guo Haifa, Lin Guihu, Huang Qi, Qiu Mantang
Department of Thoracic Surgery, Peking University People's Hospital, Beijing, China.
Department of Thoracic Surgery, Aerospace 731 Hospital, Beijing, China.
Front Oncol. 2022 Mar 31;12:843054. doi: 10.3389/fonc.2022.843054. eCollection 2022.
Circulating-free RNAs (cfRNAs) have been regarded as potential biomarkers for "liquid biopsy" in cancers. However, the circulating messenger RNA (mRNA) and long noncoding RNA (lncRNA) profiles of lung cancer have not been fully characterized. In this study, we profiled circulating mRNA and lncRNA profiles of 16 lung cancer patients and 4 patients with benign pulmonary nodules. Compared with benign pulmonary nodules, 806 mRNAs and 1,762 lncRNAs were differentially expressed in plasma of lung adenocarcinoma patients. For lung squamous cell carcinomas, 256 mRNAs and 946 lncRNAs were differentially expressed. A total of 231 mRNAs and 298 lncRNAs were differentially expressed in small cell lung cancer. Eleven mRNAs, 51 lncRNAs, and 207 canonical pathways were differentially expressed in lung cancer in total. Forty-five blood samples were collected to verify our findings performing qPCR. There are plenty of meaningful mRNAs and lncRNAs that were found. MYC, a transcription regulator associated with the stemness of cancer cells, is overexpressed in lung adenocarcinoma. Transforming growth factor beta (TGFB1), which plays pleiotropic roles in cancer progression, was found to be upregulated in lung squamous carcinoma. MALAT1, a well-known oncogenic lncRNA, was also found to be upregulated in lung squamous carcinoma. Thus, this study provided a systematic resource of mRNA and lncRNA expression profiles in lung cancer plasma.
循环游离RNA（cfRNAs）被视为癌症“液体活检”的潜在生物标志物。然而，肺癌的循环信使RNA（mRNA）和长链非编码RNA（lncRNA）图谱尚未得到充分表征。在本研究中，我们分析了16例肺癌患者和4例良性肺结节患者的循环mRNA和lncRNA图谱。与良性肺结节相比，肺腺癌患者血浆中有806种mRNA和1762种lncRNA差异表达。对于肺鳞状细胞癌，有256种mRNA和946种lncRNA差异表达。小细胞肺癌中共有231种mRNA和298种lncRNA差异表达。肺癌中总共11种mRNA、51种lncRNA和207条经典通路差异表达。采集了45份血样，通过定量聚合酶链反应（qPCR）验证我们的发现。发现了大量有意义的mRNA和lncRNA。MYC是一种与癌细胞干性相关的转录调节因子，在肺腺癌中过表达。转化生长因子β（TGFB1）在癌症进展中发挥多效性作用，在肺鳞状细胞癌中被发现上调。MALAT1是一种著名的致癌lncRNA，在肺鳞状细胞癌中也被发现上调。因此，本研究提供了肺癌血浆中mRNA和lncRNA表达谱的系统资源。
