Yu Bangrui, Peng Lanlan, Dang Wenjun, Fu Ying, Li Zhijie, Feng Jinteng, Zhao Heng, Wang Tian, Xu Feng, Yarmush Martin L, Huang Haishui
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China.
Bioinspired Engineering and Biomechanics Center (BEBC), School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China.
Bioact Mater. 2025 Apr 26;50:461-474. doi: 10.1016/j.bioactmat.2025.04.024. eCollection 2025 Aug.
Stem cell-laden hydrogel patches are promising candidates to treat chronic ulcers due to cells' long-lasting and dynamic responses to wound microenvironment. However, their clinical translations are prohibited by the cryopreservation difficulty due to their weak mechanical strength and slow biotransport capability, and by the morphological mismatch between clinical ulcers and pre-fabricated patches. Here we report a stem cell-laden alginate-dopamine hydrogel patch that can be readily cryopreserved, processed, and scaled toward clinical usages. This cell-hydrogel patch not only maintains cell viability and structure integrity during cryopreservation, but also can be directly utilized without centrifugation or incubation post cryopreservation. In addition, this tissue-adhesive hydrogel patch enables close wound contact and fast cellular response, and its scalable and flexible structure enables assembly for large or irregularly shaped ulcers. Therefore, it accelerates ulcer healing and reduces scar formation continuous, versatile, self-adjusting paracrine of imbedded, cryopreserved stem cells. These findings highlight its potential for scalable clinical applications in chronic wound management and pave the way for broader adoption of ready-to-use regenerative therapies.
由于细胞对伤口微环境具有持久且动态的反应，负载干细胞的水凝胶贴片有望成为治疗慢性溃疡的候选材料。然而，由于其机械强度较弱、生物转运能力缓慢导致的冷冻保存困难，以及临床溃疡与预制贴片之间的形态不匹配，限制了它们的临床应用。在此，我们报道了一种负载干细胞的海藻酸盐-多巴胺水凝胶贴片，它可以很容易地进行冷冻保存、加工，并扩大规模用于临床。这种细胞-水凝胶贴片不仅在冷冻保存过程中能保持细胞活力和结构完整性，而且冷冻保存后无需离心或孵育即可直接使用。此外，这种组织粘附性水凝胶贴片能够紧密贴合伤口并实现快速细胞反应，其可扩展且灵活的结构能够组装用于大型或不规则形状的溃疡。因此，它通过嵌入的、冷冻保存的干细胞持续、多功能、自我调节的旁分泌作用加速溃疡愈合并减少疤痕形成。这些发现凸显了其在慢性伤口管理中大规模临床应用的潜力，并为更广泛采用即用型再生疗法铺平了道路。
