Ntakirutimana Remy, Mujeeb Rahiman K M, Neethu K V
Centre de Recherche en Sciences Naturelles et de l'Environnement (CRSNE), University of Burundi, Bujumbura, Burundi.
School of Industrial Fisheries, Cochin University of Science and Technology, Cochin, Kerala, India.
Aquac Nutr. 2025 May 1;2025:6074225. doi: 10.1155/anu/6074225. eCollection 2025.
This study evaluated the effects of a commercial probiotic containing and on the growth performance, intestinal histological structure, body composition, hematology, and microbiota of red tilapia. Fingerlings were fed four different diets: a control diet (Pd0) and three diets (Pd1, Pd2, and Pd3) containing 15, 20, and 40 mL of probiotics/kg, respectively, for 12 weeks. Probiotic supplementation had no significant effect on water quality parameters. Compared with the control diet, all the probiotic diets improved growth performance, with greater final body weight (FBW), net weight gain (NWG), weight gain, average daily weight gain (ADWG), specific growth rate (SGR), and feed conversion efficiency (FCE). The feed conversion ratio (FCR) was lower in all probiotic-treated fish compared to control. The survival rate was also higher in the probiotic groups, though the difference was not significant. There was no significant difference in crude ash or lipid contents. However, protein content was significantly higher in Pd2 and Pd3, while moisture content (MC) was significantly higher in Pd3 than in the control group. Histological examination revealed increased villi length and width, being significantly higher in Pd2 and Pd3, while significantly greater muscular thickness and intestinal diameter were observed in Pd3-treated fish. These values increased with probiotic dose. The intestinal total viable count (TVC) was the highest in Pd2 and the lowest in the control group. The water TVC was the highest in Pd3 and the lowest in Pd0. The number of spp. in the intestine and culture water increased with probiotic dose, while intestinal and culture water counts decreased. Hematological analysis showed significant increases in red blood cell (RBC) count, hematocrit, mean corpuscular hemoglobin concentration (MCHC), and hemoglobin (Hb) in the treated groups compared with the control. The incorporation of and at 40 mL/kg in red tilapia diets improved growth performance, intestinal health, and general welfare.
本研究评估了一种含有[具体成分未给出]和[具体成分未给出]的商业益生菌对红罗非鱼生长性能、肠道组织结构、身体组成、血液学和微生物群的影响。将幼鱼投喂四种不同的饲料：对照饲料（Pd0）和三种分别含有15、20和40毫升/千克益生菌的饲料（Pd1、Pd2和Pd3），持续12周。添加益生菌对水质参数没有显著影响。与对照饲料相比，所有添加益生菌的饲料均改善了生长性能，最终体重（FBW）、净重增加（NWG）、体重增加、平均日增重（ADWG）、特定生长率（SGR）和饲料转化效率（FCE）更高。与对照相比，所有经益生菌处理的鱼的饲料转化率（FCR）更低。益生菌组的存活率也更高，尽管差异不显著。粗灰分或脂质含量没有显著差异。然而，Pd2和Pd3组的蛋白质含量显著更高，而Pd3组的水分含量（MC）显著高于对照组。组织学检查显示绒毛长度和宽度增加，Pd2和Pd3组显著更高，而在经Pd3处理的鱼中观察到肌肉厚度和肠直径显著更大。这些值随益生菌剂量增加。肠道总活菌数（TVC）在Pd2组最高，对照组最低。水体TVC在Pd3组最高，Pd0组最低。肠道和养殖水中[具体菌属未给出]的数量随益生菌剂量增加，而肠道和养殖水中[具体菌属未给出]数量减少。血液学分析表明，与对照组相比，处理组的红细胞（RBC）计数、血细胞比容、平均红细胞血红蛋白浓度（MCHC）和血红蛋白（Hb）显著增加。在红罗非鱼饲料中添加40毫升/千克的[具体成分未给出]和[具体成分未给出]可改善生长性能、肠道健康和总体健康状况。
