Gao Yang-Yang, Yang Chang-Xin, Wu Hong, Li Jian-Hong, Wen Ting, Wang Wei, Wang Zhi-Zheng, Chen Hui-Min, Pei Rong-Jie, Huang Zhi-You, Zheng Yu-Guo, Yang Guang-Fu, Li Xiang-Yang, Hao Ge-Fei
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, 550025, P. R. China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430072, P. R. China.
Adv Sci (Weinh). 2025 Jun;12(22):e2417212. doi: 10.1002/advs.202417212. Epub 2025 May 8.
Frequent exposure to environmental stresses can potentially reduce plant yield by up to 60%. The regulation of abscisic acid (ABA) signaling, especially its core receptors, is crucial for the adaptation of plants to environmental stress. However, efficient identification of ABA-signaling modulators remains a challenge. Here, a global high-throughput fluorescence screening (GhitFluors) platform is established to efficiently identify ABA signaling modulators. The GhitFluors platform yields a similar tendency for binding affinity to that determined by classical methods. Diopyridin is effectively identified, a regulator with strong binding affinity to Pyrabactin Resistance 1 (PYR1), and more than 80% inhibitory activity against HAB1, which is equivalent to ABA. Additionally, it can also effectively mitigate the adverse effects of drought on plants. The findings promise the start of a new era for achieving high-efficiency identification ABA signaling modulators.
频繁暴露于环境胁迫下可能会使植物产量降低多达60%。脱落酸（ABA）信号传导的调控，尤其是其核心受体，对于植物适应环境胁迫至关重要。然而，高效鉴定ABA信号调节剂仍然是一项挑战。在此，建立了一个全球高通量荧光筛选（GhitFluors）平台以高效鉴定ABA信号调节剂。GhitFluors平台产生的结合亲和力趋势与经典方法测定的趋势相似。二吡啶被有效鉴定出来，它是一种与抗脱落酸1（PYR1）具有强结合亲和力的调节剂，对HAB1具有超过80%的抑制活性，这与ABA相当。此外，它还能有效减轻干旱对植物的不利影响。这些发现预示着实现高效鉴定ABA信号调节剂的新时代的开始。
