Biodegradation of ciprofloxacin by a manganese-oxidizing fungus Cladosporium sp. XM01: Performance and transcriptome analysis.

Biogenic manganese (Mn) oxidation presents a promising approach for ciprofloxacin (CIP) removal from wastewater, yet the interaction between Mn bio-oxidation and CIP degradation remains unclear. The Mn-oxidizing fungus Cladosporium sp. XM01 was selected as a model strain in this study, to explore the impacts of CIP on microbial growth, function and biotransformation. Results showed that CIP exhibited a promotive effect on the growth and Mn(II) oxidation capacity of XM01. After 192 h of cultivation, 39.80 %-69.19 % of CIP was removed by XM01 in the absence of Mn(II), while over 84 % was removed with 300 μM Mn(II), demonstrating both direct and Mn(II)-enhanced indirect degradation of CIP. Transcriptomic analysis revealed that the upregulation of ribosome, peroxisome, and tyrosine metabolism pathways enhanced XM01's adaptation to CIP and supported microbial growth. Cytochrome P450 (CYP450) enzymes were implicated as key mediators in CIP degradation. Additionally, in the presence of Mn(II), the further upregulation of transmembrane transporters, NAD(P)H dehydrogenase, and CYP450 indicated that Mn bio-oxidation enhanced XM01's adaptation and response to CIP, thereby accelerating its degradation. Proposed CIP degradation pathways include piperazine epoxidation, decarboxylation, and hydroxylation. This study advances the understanding of fungal Mn oxidation in antibiotic removal, emphasizing its potential in wastewater treatment.