Wang Mei, Xu Zuxin, Huang Yangrui, Dong Bin
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Ministry of Education Key Laboratory of Yangtze River Water Environment, Tongji University, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Ministry of Education Key Laboratory of Yangtze River Water Environment, Tongji University, Shanghai 200092, China.
J Hazard Mater. 2025 Aug 15;494:138543. doi: 10.1016/j.jhazmat.2025.138543. Epub 2025 May 8.
Biogenic manganese (Mn) oxidation presents a promising approach for ciprofloxacin (CIP) removal from wastewater, yet the interaction between Mn bio-oxidation and CIP degradation remains unclear. The Mn-oxidizing fungus Cladosporium sp. XM01 was selected as a model strain in this study, to explore the impacts of CIP on microbial growth, function and biotransformation. Results showed that CIP exhibited a promotive effect on the growth and Mn(II) oxidation capacity of XM01. After 192 h of cultivation, 39.80 %-69.19 % of CIP was removed by XM01 in the absence of Mn(II), while over 84 % was removed with 300 μM Mn(II), demonstrating both direct and Mn(II)-enhanced indirect degradation of CIP. Transcriptomic analysis revealed that the upregulation of ribosome, peroxisome, and tyrosine metabolism pathways enhanced XM01's adaptation to CIP and supported microbial growth. Cytochrome P450 (CYP450) enzymes were implicated as key mediators in CIP degradation. Additionally, in the presence of Mn(II), the further upregulation of transmembrane transporters, NAD(P)H dehydrogenase, and CYP450 indicated that Mn bio-oxidation enhanced XM01's adaptation and response to CIP, thereby accelerating its degradation. Proposed CIP degradation pathways include piperazine epoxidation, decarboxylation, and hydroxylation. This study advances the understanding of fungal Mn oxidation in antibiotic removal, emphasizing its potential in wastewater treatment.
生物源锰（Mn）氧化是一种从废水中去除环丙沙星（CIP）的很有前景的方法，但锰生物氧化与CIP降解之间的相互作用仍不清楚。本研究选择锰氧化真菌枝孢菌属XM01作为模型菌株，以探讨CIP对微生物生长、功能和生物转化的影响。结果表明，CIP对XM01的生长和Mn（II）氧化能力具有促进作用。培养192小时后，在不存在Mn（II）的情况下，XM01去除了39.80%-69.19%的CIP，而在添加300μM Mn（II）的情况下，去除率超过84%，表明CIP存在直接降解和Mn（II）增强的间接降解。转录组分析表明，核糖体、过氧化物酶体和酪氨酸代谢途径的上调增强了XM01对CIP的适应性并支持微生物生长。细胞色素P450（CYP450）酶被认为是CIP降解的关键介质。此外，在存在Mn（II）的情况下，跨膜转运蛋白、NAD（P）H脱氢酶和CYP450的进一步上调表明锰生物氧化增强了XM01对CIP的适应性和反应，从而加速了其降解。推测的CIP降解途径包括哌嗪环氧化、脱羧和羟基化。本研究增进了对真菌锰氧化在抗生素去除方面的理解，强调了其在废水处理中的潜力。
