Stefanick M L, Smith E R, Szumowski D A, Davidson J M
Pharmacol Biochem Behav. 1985 Jul;23(1):55-63. doi: 10.1016/0091-3057(85)90130-3.
The effect of guanethidine, an adrenergic neuron blocking agent, on sexual behavior, penile reflexes, and spontaneous seminal emission (SSE) in the rat was studied by acute (i.e., 4 hours prior to testing) and daily IP injection of a low (5 mg/kg) and moderately high (25 mg/kg) dose of the drug. Acute low dose treatment eliminated the expulsion of a seminal plug with behavioral ejaculation without affecting sexual behavior; while acute high dose administration significantly decreased the number of intromissions preceding ejaculation and eliminated emission in copula and SSE for 3 days, with no evidence of retrograde ejaculation. Acute high dose treatment also increased the number of long flips displayed in the penile reflex test, and resulted in significant depression in plasma testosterone (T) and luteinizing hormone (LH) levels. Daily injection with the low dose eliminated emission in and ex copula for 4 weeks, without altering sexual behavior or penile reflexes. Seminal emission in copula reappeared more rapidly after stopping injections than SSE. Chronic high dose treatment was also without effect on copulatory activity. There was a partial recovery of emission in copula by the fourth week of treatment, suggesting that a nonadrenergic mechanism had assumed this function. In penile reflex tests conducted after 4 and 8 weeks, significantly fewer erections were displayed by drug-treated animals. During the period of initial recovery of emission in copula, emission during the reflex test was markedly increased, but SSE was decreased. Plasma T was significantly suppressed after two and four weeks of daily injections, but not thereafter, while plasma LH levels were not affected by the drug.
研究了肾上腺素能神经元阻断剂胍乙啶对大鼠性行为、阴茎反射和自发性射精（SSE）的影响，通过急性（即测试前4小时）和每日腹腔注射低剂量（5mg/kg）和中等高剂量（25mg/kg）的该药物进行研究。急性低剂量治疗消除了伴有行为射精的精液栓排出，而不影响性行为；而急性高剂量给药显著减少射精前的插入次数，并在3天内消除交配时的射精和SSE，无逆行射精的证据。急性高剂量治疗还增加了阴茎反射试验中长翻转的次数，并导致血浆睾酮（T）和黄体生成素（LH）水平显著降低。每日注射低剂量在4周内消除了交配时和交配后的射精，而不改变性行为或阴茎反射。停止注射后，交配时的射精比SSE更快重新出现。慢性高剂量治疗对交配活动也无影响。治疗第四周时，交配时射精部分恢复，表明非肾上腺素能机制承担了这一功能。在4周和8周后进行的阴茎反射试验中，药物治疗的动物勃起次数明显减少。在交配时射精初步恢复期间，反射试验期间的射精明显增加，但SSE减少。每日注射两周和四周后，血浆T显著受到抑制，但此后未受影响，而血浆LH水平不受该药物影响。
