Arredondo Daniela, Grecco Sofia, Panzera Yanina, Zunino Pablo, Antúnez Karina
Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay.
Centro de Investigación en Ciencias Ambientales (CICA), Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay.
Environ Microbiol Rep. 2025 Jun;17(3):e70097. doi: 10.1111/1758-2229.70097.
Honey bees (Apis mellifera) play a crucial role in global food production through pollination services. However, their populations are threatened by various stressors, like the ectoparasitic mite Varroa destructor and associated viral pathogens. In this study, we aimed to characterise and compare the viral communities (viromes) in V. destructor-resistant and susceptible colonies using high-throughput sequencing. Our findings revealed differences in virome composition associated with the season and not with the resistance or susceptibility to V. destructor. Furthermore, we detected Apis mellifera filamentous virus (AmFV) and Lake Sinai virus (LSV) for the first time in Uruguay, and obtained the complete or partial genomes of both viruses, along with those of other previously described viruses, such as Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Deformed wing virus (DWV), and Sacbrood virus (SBV). This study contributes to a deeper understanding of the virome dynamics in honey bees. It highlights the importance of this type of study for the early detection of new viral pathogens, which could help to understand the tripartite network involving V. destructor, honey bees, and viruses.
蜜蜂（西方蜜蜂）通过授粉服务在全球粮食生产中发挥着至关重要的作用。然而，它们的种群受到各种压力因素的威胁，如外寄生螨狄斯瓦螨和相关的病毒病原体。在本研究中，我们旨在利用高通量测序对狄斯瓦螨抗性和易感蜂群中的病毒群落（病毒组）进行表征和比较。我们的研究结果揭示了与季节相关而非与对狄斯瓦螨的抗性或易感性相关的病毒组组成差异。此外，我们首次在乌拉圭检测到西方蜜蜂丝状病毒（AmFV）和西奈湖病毒（LSV），并获得了这两种病毒以及其他先前描述的病毒（如急性蜜蜂麻痹病毒（ABPV）、黑蜂王台病毒（BQCV）、残翅病毒（DWV）和囊状幼虫病毒（SBV））的完整或部分基因组。本研究有助于更深入地了解蜜蜂病毒组的动态变化。它强调了这类研究对于新病毒病原体早期检测的重要性，这有助于理解涉及狄斯瓦螨、蜜蜂和病毒的三方网络。
