Qian Luomeng, Zhang Sihe
Department of Cell Biology, School of Medicine, Nankai University, Tianjin, China.
Methods Mol Biol. 2025;2917:3-10. doi: 10.1007/978-1-0716-4478-2_1.
Gelatin zymography is a simple semiquantitative technique for assaying gelatinases in various biological samples. It is particularly feasible to measure two gelatin-degrading matrix metalloproteinases, MMP-2 (gelatinase A) and MMP-9 (gelatinase B). The use of SDS-polyacrylamide gels impregnated with gelatin provides a reliable way to detect and quantify the type, amount, and activation level of gelatinases in biological samples (cell lysates, tissue extracts, and biological fluids). This method can be used to investigate the expression profile and activation level of gelatinase in cancer cells or tissues and to determine how these changes are related to cancer progression. However, it is crucial to understand the principle and limitation of gelatin zymography before interpreting the experimental data. Gelatin zymography is a powerful method for assaying gelatinases in cancer research if some precautions are taken.
明胶酶谱法是一种用于检测各种生物样品中明胶酶的简单半定量技术。测量两种降解明胶的基质金属蛋白酶，即基质金属蛋白酶-2（明胶酶A）和基质金属蛋白酶-9（明胶酶B）尤其可行。使用浸渍有明胶的SDS-聚丙烯酰胺凝胶为检测和定量生物样品（细胞裂解物、组织提取物和生物体液）中明胶酶的类型、数量和激活水平提供了一种可靠的方法。该方法可用于研究癌细胞或组织中明胶酶的表达谱和激活水平，并确定这些变化与癌症进展的关系。然而，在解释实验数据之前，了解明胶酶谱法的原理和局限性至关重要。如果采取一些预防措施，明胶酶谱法是癌症研究中检测明胶酶的一种强大方法。
