明胶酶谱法：一种检测明胶酶的简单有效技术。

Gelatin Zymography: A Simple and Effective Technique to Detect Gelatinases.

作者信息

Xue Meilang, Lin Haiyan, Jackson Christer J, March Lyn

机构信息

Sutton Arthritis Research Laboratory, Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.

The Australian Arthritis and Autoimmune Biobank Collaborative (A3BC), Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.

出版信息

Methods Mol Biol. 2025;2917:29-39. doi: 10.1007/978-1-0716-4478-2_3.

DOI:10.1007/978-1-0716-4478-2_3

PMID:40347329

Abstract

Matrix metalloproteinases (MMPs) are zinc-dependent enzymes capable of breaking down all extracellular matrix (ECM) components. The gelatinases are a subgroup of MMPs which have two members: gelatinase A (MMP-2) and gelatinase B (MMP-9). They are highly efficient in breaking down denatured collagen I or gelatin. This ability of gelatinases has led to the development of a technique called gelatin zymography. This technique provides a simple, sensitive, and robust way to detect gelatinolytic activity in biological samples. This chapter provides a detailed protocol on how to implement the gelatin zymography and analyze gelatinases in biological samples using this technique.

摘要

基质金属蛋白酶（MMPs）是一类依赖锌的酶，能够分解所有细胞外基质（ECM）成分。明胶酶是MMPs的一个亚组，有两个成员：明胶酶A（MMP-2）和明胶酶B（MMP-9）。它们在分解变性的I型胶原蛋白或明胶方面效率极高。明胶酶的这种能力导致了一种称为明胶酶谱法的技术的发展。该技术提供了一种简单、灵敏且可靠的方法来检测生物样品中的明胶酶活性。本章提供了一份详细的方案，介绍如何实施明胶酶谱法，并使用该技术分析生物样品中的明胶酶。