用酶谱法检测基质金属蛋白酶
Detection of Matrix Metalloproteinases by Zymography.
作者信息
Tajhya Rajeev B, Patel Rutvik S, Beeton Christine
机构信息
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
出版信息
Methods Mol Biol. 2017;1579:231-244. doi: 10.1007/978-1-4939-6863-3_12.
Abstract
Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis. They are also involved in the pathogenesis of a vast array of diseases, including but not limited to systemic inflammation, various cancers, and cardiovascular, neurological, and autoimmune diseases. Here, we describe gel zymography to detect MMPs in cell and tissue samples and in cell culture supernatants.
摘要
基质金属蛋白酶（MMPs）是20多种含锌的内肽酶，可切割内部肽键，导致蛋白质降解。它们在许多生理细胞功能中发挥关键作用，包括组织重塑、胚胎发生和血管生成。它们还参与了大量疾病的发病机制，包括但不限于全身炎症、各种癌症以及心血管、神经和自身免疫性疾病。在此，我们描述了用于检测细胞和组织样本以及细胞培养上清液中MMPs的凝胶酶谱法。
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J Clin Periodontol. 2016 Jan;43(1):34-45. doi: 10.1111/jcpe.12479. Epub 2016 Feb 12.
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