Rippon J W, Anderson D N, Jacobsohn S, Soo Hoo M, Garber E D
Mycopathologia. 1977 Feb 18;60(2):65-72. doi: 10.1007/BF00490374.
In a study of sera from patients with proven or suspected blastomycosis, positive immunodiffusion tests were obtained in all active cases when fresh sera were tested with a cell sap (CS) antigen. False negatives occurred on occasion when an ethanol precipitate (EPF) antigen was used alone. No false positives were found. The CS antigen from the (+) mating type had in common two lines of identity with the (CS) antigen of the (-) type. In addition, other lines were present when the patient was infected with the same mating type as was used for the preparation of the antigen. No differences in the electrophoretic patterns of the enzymes leucine amino peptidase or phosphatase were noted when preparations from the two mating types were compared. However, a distinct pattern was noted when the alpha esterases of the (+) and (-) mating types were examined. Specific alpha esterase antibodies were present in patients' sera.
在一项针对已确诊或疑似芽生菌病患者血清的研究中，当用细胞液（CS）抗原检测新鲜血清时，所有活动期病例的免疫扩散试验均呈阳性。单独使用乙醇沉淀物（EPF）抗原时偶尔会出现假阴性。未发现假阳性。来自（+）交配型的CS抗原与（-）型的（CS）抗原共有两条相同的沉淀线。此外，当患者感染的交配型与用于制备抗原的交配型相同时，还会出现其他沉淀线。比较两种交配型的制剂时，未发现亮氨酸氨基肽酶或磷酸酶的电泳图谱有差异。然而，在检查（+）和（-）交配型的α酯酶时，发现了明显的图谱差异。患者血清中存在特异性α酯酶抗体。
