Sekhon A S, Kaufman L, Kobayashi G S, Moledina N H, Jalbert M
National Centre for Human Mycotic Diseases, Provincial Laboratory of Public Health, University of Alberta Hospitals, Edmonton, Canada.
J Med Vet Mycol. 1995 Mar-Apr;33(2):123-5.
One hundred and three sera drawn from 20 proven and 65 suspected cases of blastomycosis were examined concurrently with the enzymes immunoassay and microimmunodiffusion tests for the 'A' antibody specific for Blastomyces dermatitidis. Results indicated that all 20 proven sera were positive by both these tests. Thirteen of the 65 sera from suspected blastomycosis cases were positive by the enzyme immunoassay only, whereas none reacted positively in the micro-immunodiffusion test. Eighteen sera from apparently normal subjects, and patients with heterologous fungal and HIV infections were also tested by both tests. The sensitivity and specificity of the enzyme immunoassay test was 100% and 85.6%, respectively. The micro- immunodiffusion test was 100% sensitive and specific. In light of the fact that the enzyme immunoassay test is not entirely specific, a positive result should be confirmed by either a positive culture, histopathology or micro-immunodiffusion test.
从20例已确诊和65例疑似芽生菌病患者中采集了103份血清，同时采用酶免疫测定法和微量免疫扩散试验检测针对皮炎芽生菌的“A”抗体。结果表明，所有20份已确诊血清在这两种检测中均呈阳性。65份疑似芽生菌病病例的血清中，有13份仅通过酶免疫测定法呈阳性，而在微量免疫扩散试验中均无阳性反应。还对18份来自明显正常受试者以及患有异种真菌感染和艾滋病毒感染患者的血清进行了这两种检测。酶免疫测定法的敏感性和特异性分别为100%和85.6%。微量免疫扩散试验的敏感性和特异性均为100%。鉴于酶免疫测定法并非完全特异，阳性结果应由阳性培养、组织病理学或微量免疫扩散试验加以证实。
