Yang Yong-Min, Chai Lian-Jing, Zhang Rui-Feng, Fan Yu-Juan, Mao Chen-Xue, Ding Ai-Lian
Kunming Kingmed Institute for Clinical Laboratory Co., Ltd. kunming, Yunnan province, PR China.
Dehong Prefecture People's Hospital, Dehong, Yunnan province, PR China.
Diagn Microbiol Infect Dis. 2025 Sep;113(1):116892. doi: 10.1016/j.diagmicrobio.2025.116892. Epub 2025 May 3.
We report a case of a Chinese male patient with a history of riverside sampling work who presented with a cough and sputum production. Chest computed tomography revealed multiple lesions in both lungs, and bronchial examination indicated inflammatory changes. Histoplasmosis was identified through targeted next-generation sequencing (tNGS)detection of bronchoalveolar lavage fluid and diagnosed as Histoplasma capsulatum pneumonia. Lung biopsy and mNGS test further confirmed that the pathogen causing the pulmonary infection was Histoplasma capsulatum. Finally, the lesions were reduced and improved after three months of oral itraconazole and fluconazole (Diflucan) treatment. This case report highlights the advantages of tNGS technology, including rapid detection, broad coverage, high accuracy, and low cost. Therefore, tNGS can be a valuable supplement for diagnosing histoplasmosis and other pathogens.
我们报告了一例中国男性患者，该患者有河边采样工作史，出现咳嗽和咳痰症状。胸部计算机断层扫描显示双肺有多个病灶，支气管检查提示有炎症改变。通过对支气管肺泡灌洗液进行靶向二代测序（tNGS）检测确诊为组织胞浆菌病，并诊断为荚膜组织胞浆菌肺炎。肺活检和宏基因组二代测序（mNGS）检测进一步证实引起肺部感染的病原体为荚膜组织胞浆菌。最后，经口服伊曲康唑和氟康唑（大扶康）治疗三个月后，病灶缩小并有所改善。本病例报告突出了tNGS技术的优势，包括检测快速、覆盖范围广、准确性高和成本低。因此，tNGS可作为诊断组织胞浆菌病和其他病原体的有价值补充手段。
