AP2/ERF 转录因子 MdDREB2A 调控干旱胁迫下氮素利用和蔗糖运输。

The AP2/ERF transcription factor MdDREB2A regulates nitrogen utilisation and sucrose transport under drought stress.

机构信息

Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, Shandong, China.

Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilisation, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi, Xinjiang, China.

出版信息

Plant Cell Environ. 2024 May;47(5):1668-1684. doi: 10.1111/pce.14834. Epub 2024 Jan 28.

DOI:10.1111/pce.14834

PMID:38282271

Abstract

Drought stress is one of the main environmental factors limiting plant growth and development. Plants adapt to changing soil moisture by modifying root architecture, inducing stomatal closure, and inhibiting shoot growth. The AP2/ERF transcription factor DREB2A plays a key role in maintaining plant growth in response to drought stress, but the molecular mechanism underlying this process remains to be elucidated. Here, it was found that overexpression of MdDREB2A positively regulated nitrogen utilisation by interacting with DRE cis-elements of the MdNIR1 promoter. Meanwhile, MdDREB2A could also directly bind to the promoter of MdSWEET12, which may enhance root development and nitrogen assimilation, ultimately promoting plant growth. Overall, this regulatory mechanism provides an idea for plants in coordinating with drought tolerance and nitrogen assimilation to maintain optimal plant growth and development under drought stress.

摘要

干旱胁迫是限制植物生长和发育的主要环境因素之一。植物通过改变根系结构、诱导气孔关闭和抑制地上部生长来适应土壤水分的变化。AP2/ERF 转录因子 DREB2A 在维持植物生长以响应干旱胁迫方面起着关键作用，但这一过程的分子机制仍有待阐明。在这里，研究发现，过表达 MdDREB2A 通过与 MdNIR1 启动子的 DRE 顺式元件相互作用，正向调节氮素利用。同时，MdDREB2A 也可以直接结合 MdSWEET12 的启动子，这可能增强根的发育和氮的同化，最终促进植物生长。总的来说，这种调控机制为植物协调耐旱性和氮同化提供了一种思路，以在干旱胁迫下维持最佳的植物生长和发育。