Safitri Azizah H, Sayyida Rahmata A, Setyawan Sigit, Tyagita Nurina
Department of Biochemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia.
Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia.
Narra J. 2025 Apr;5(1):e1995. doi: 10.52225/narra.v5i1.1995. Epub 2025 Feb 13.
Aging is a predominant risk factor for several diseases associated with reduced life expectancy. To address this risk factor, several studies have proposed the combined use of porang glucomannan and a high-protein diet to improve various aging markers. The aim of this study was to determine the effects of porang glucomannan and high-protein combination diet as an anti-aging agent. An experimental study using a post-test-only control group design was conducted using Sprague Dawley white rats. The animals were randomly divided into four groups with different treatments: normal control, D-galactose, high-protein diet, and a combination of porang glucomannan and high-protein combination diet. Blood samples were then collected from the ophthalmic vein on day 58 for biomarker measurement using the enzyme-linked immunosorbent assay (ELISA) method. The parameters measured were superoxide dismutase (SOD), malondialdehyde (MDA), interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), insulin growth factor-1 (IGF-1), NOD-like receptor family pyrin domain-containing protein 3 (NLRP3), growth differentiation factor-11 (GDF11), and α-Klotho levels. The results showed that the combination of porang glucomannan and high-protein diet could improve oxidative stress, inflammation, and aging markers. The analysis of variance (ANOVA) test followed by post-hoc showed significant differences between the combination diet and high protein group ( < 0.001). In addition, the average levels of oxidative stress markers (SOD and MDA) in porang glucomannan and high-protein combination group were improved significantly. Similar results were also obtained for inflammatory markers (IL-6 and TNF-α) and aging markers (NLRP3, IGF-1, GDF-11, and α-Klotho). The mean NRLP-3 levels in glucomannan and high-protein combination group were not significantly different compared to control. The study highlights that the combination of porang glucomannan and a high-protein diet effectively improved various aging markers.
衰老是几种与预期寿命缩短相关疾病的主要风险因素。为应对这一风险因素，多项研究提出联合使用茯苓葡甘露聚糖和高蛋白饮食来改善各种衰老标志物。本研究的目的是确定茯苓葡甘露聚糖与高蛋白组合饮食作为抗衰老剂的效果。采用仅后测对照组设计的实验研究，以斯普拉格-道利白色大鼠为实验对象。将动物随机分为四组，给予不同处理：正常对照组、D-半乳糖组、高蛋白饮食组以及茯苓葡甘露聚糖与高蛋白组合饮食组。然后在第58天从眼静脉采集血样，采用酶联免疫吸附测定（ELISA）法进行生物标志物检测。检测的参数包括超氧化物歧化酶（SOD）、丙二醛（MDA）、白细胞介素（IL）-6、肿瘤坏死因子-α（TNF-α）、胰岛素生长因子-1（IGF-1）、NOD样受体家族含pyrin结构域蛋白3（NLRP3）、生长分化因子-11（GDF11）和α-klotho水平。结果表明，茯苓葡甘露聚糖与高蛋白饮食的组合可改善氧化应激、炎症和衰老标志物。方差分析（ANOVA）检验及事后检验显示，组合饮食组与高蛋白组之间存在显著差异（<0.001）。此外，茯苓葡甘露聚糖与高蛋白组合组的氧化应激标志物（SOD和MDA）平均水平显著改善。炎症标志物（IL-6和TNF-α）和衰老标志物（NLRP3、IGF-1、GDF-11和α-klotho）也得到了类似结果。与对照组相比，葡甘露聚糖与高蛋白组合组的平均NRLP-3水平无显著差异。该研究强调，茯苓葡甘露聚糖与高蛋白饮食的组合有效改善了各种衰老标志物。
