Nurliyani Nurliyani, Harmayani Eni, Sunarti Sunarti
Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Kampus UGM, Bulaksumur, Yogyakarta 55281, Indonesia.
Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora 1 Bulaksumur, Yogyakarta 55281, Indonesia.
Vet World. 2022 Jan;15(1):173-181. doi: 10.14202/vetworld.2022.173-181. Epub 2022 Jan 28.
Kefir, a natural probiotic containing bacteria and yeast, is a fermented milk product, whereas glucomannan from porang tuber () is prebiotic . Simvastatin is a potent lipid-lowering statin that can be utilized for pharmacological therapy in obesity. This study aimed to determine the effect of goat milk kefir supplemented with porang glucomannan (synbiotic kefir) and goat milk kefir without glucomannan (probiotic kefir) on blood glucose, hemoglobin A1c (HbA1c), free fatty acids (FFAs), tumor necrosis factor-alpha (TNF-α), gene expression of peroxisome proliferator-activated receptor gamma (PPARg), and insulin-producing cells in rats fed a high-fat and high-fructose (HFHF) diet.
Male Sprague-Dawley rats were divided into five dietary groups: (1) Normal control, (2) rats fed HFHF, (3) rats fed HFHF+probiotic kefir, (4) rats fed HFHF+synbiotic kefir, and (5) rats fed HFHF+simvastatin. All of these treatments were administered for 4 weeks.
There were no significant differences in plasma glucose levels in HFHF diet-fed rats before and after treatment. However, plasma HbA1c and TNF-α decreased, and FFAs were inhibited in rats after treatment with synbiotic kefir. Synbiotic kefir decreased the gene expression of PPARγ2 in HFHF diet-fed rats but did not affect the total number of islets of Langerhans and insulin-producing cells.
Synbiotic kefir improved the health of rats fed an HFHF diet by decreasing HbA1c, TNF-α, and PPARγ2 gene expression and preventing an increase in FFAs.
开菲尔是一种含有细菌和酵母的天然益生菌发酵乳制品，而来自薯蓣属块茎的葡甘露聚糖是益生元。辛伐他汀是一种有效的降脂他汀类药物，可用于肥胖症的药物治疗。本研究旨在确定添加薯蓣属葡甘露聚糖的山羊奶开菲尔（合生元开菲尔）和不添加葡甘露聚糖的山羊奶开菲尔（益生菌开菲尔）对高脂高果糖（HFHF）饮食喂养大鼠的血糖、糖化血红蛋白（HbA1c）、游离脂肪酸（FFA）、肿瘤坏死因子-α（TNF-α）、过氧化物酶体增殖物激活受体γ（PPARg）基因表达以及胰岛素生成细胞的影响。
雄性Sprague-Dawley大鼠分为五个饮食组：（1）正常对照组，（2）HFHF饮食喂养组，（3）HFHF饮食+益生菌开菲尔喂养组，（4）HFHF饮食+合生元开菲尔喂养组，（5）HFHF饮食+辛伐他汀喂养组。所有这些处理均持续4周。
HFHF饮食喂养大鼠治疗前后血浆葡萄糖水平无显著差异。然而，合生元开菲尔治疗后大鼠的血浆HbA1c和TNF-α降低，FFA受到抑制。合生元开菲尔降低了HFHF饮食喂养大鼠中PPARγ2的基因表达，但不影响胰岛和胰岛素生成细胞的总数。
合生元开菲尔通过降低HbA1c、TNF-α和PPARγ2基因表达以及防止FFA增加，改善了HFHF饮食喂养大鼠的健康状况。
