Salinas-Nolasco Cristina, Pérez-Hernández Elizabeth, Garza Secilia, Park Hui Gyu, Brenna J Thomas, Castañeda-Hernández Gilberto, Reyes-López César A S, Pérez-Hernández Nury, Chávez-Piña Aracely Evangelina
Laboratorio de Farmacología, Doctorado en Ciencias en Biotecnología, Ciudad de México 07320, México.
Escuela Nacional de Medicina y Homeopatía del Instituto Politécnico Nacional, Ciudad de México 07320, México.
Prev Nutr Food Sci. 2025 Apr 30;30(2):132-140. doi: 10.3746/pnf.2025.30.2.132.
Polyunsaturated fatty acids (PUFAs) are known to have beneficial effects. In particular, the consumption of omega-3 PUFAs has recently increased because of their effects on human health. Previous studies have investigated the activity of alpha-linolenic acid (ALA; C:18 omega-3) in metabolism and inflammation models. In a murine model of colitis, treatment with ALA effectively reduced inflammation. Previously, our research group identified the protective action of docosahexaenoic acid against gastric damage caused by nonsteroidal anti-inflammatory drugs. The present study aimed to examine the impact of ALA in an indomethacin-induced gastric injury model and to determine its antioxidant activity in gastric tissue. Female Wistar rats were administered ALA over 10 days (20 mg/kg, orally). Two hours after the final ALA administration, the rats were given indomethacin (30 mg/kg, orally) to induce gastric injury. After 3 h, the rats were euthanized, and each stomach lesion was measured to determine the total damage. Stomach tissue samples were collected for the analysis of various antioxidant indicators. The results show ALA's gastroprotective effect following 10-day administration. ALA treatment significantly reduced gastric reactive oxygen species and malondialdehyde levels in the indomethacin-induced injury group. Moreover, ALA treatment decreased the levels of nitric oxide, myeloperoxidase, leukotriene B, and increased glutathione following indomethacin administration. These results suggest that the gastroprotective effects of ALA are likely attributed to its role in the antioxidant pathway in indomethacin-induced gastric injury.
多不饱和脂肪酸（PUFAs）具有有益作用。特别是，由于ω-3多不饱和脂肪酸对人类健康的影响，其消费量最近有所增加。先前的研究已经在代谢和炎症模型中研究了α-亚麻酸（ALA；C：18 ω-3）的活性。在结肠炎小鼠模型中，用ALA治疗可有效减轻炎症。此前，我们的研究小组确定了二十二碳六烯酸对非甾体抗炎药引起的胃损伤的保护作用。本研究旨在研究ALA在吲哚美辛诱导的胃损伤模型中的作用，并确定其在胃组织中的抗氧化活性。对雌性Wistar大鼠连续10天口服ALA（20 mg/kg）。在最后一次给予ALA两小时后，给大鼠口服吲哚美辛（30 mg/kg）以诱导胃损伤。3小时后，对大鼠实施安乐死，并测量每个胃部病变以确定总损伤程度。收集胃组织样本以分析各种抗氧化指标。结果显示，连续10天给予ALA具有胃保护作用。在吲哚美辛诱导的损伤组中，ALA治疗显著降低了胃活性氧和丙二醛水平。此外，ALA治疗降低了吲哚美辛给药后一氧化氮、髓过氧化物酶、白三烯B的水平，并提高了谷胱甘肽水平。这些结果表明，ALA的胃保护作用可能归因于其在吲哚美辛诱导的胃损伤抗氧化途径中的作用。
