Meng Zhuolun, White Liam, Xie Pengfei, Raji Hassan, Mahmoodi S Reza, Karapiperis Aris, Lin Hao, Drazer German, Javanmard Mehdi, DeMauro Edward P
Department of Electrical and Computer Engineering, Rutgers University-New Brunswick Piscataway NJ 08854 USA
Department of Mechanical and Aerospace Engineering, Rutgers University-New Brunswick Piscataway NJ 08854 USA
Sens Diagn. 2025 Apr 30. doi: 10.1039/d5sd00002e.
This work explores label-free biosensing as an effective method for biomolecular analysis, ensuring the preservation of native conformation and biological activity. The focus is on a novel electronic biosensing platform utilizing micro-fabricated nanowell-based impedance sensors, offering rapid, point-of-care diagnosis for SARS-CoV-2 (COVID-19) detection. The nanowell sensor, constructed on a silica substrate through a series of microfabrication processes including deposition, patterning, and etching, features a 5 × 5 well array functionalized with antibodies. Real-time impedance changes within the nanowell array enable diagnostic results within ten minutes using small sample volumes (<5 μL). The research includes assays for SARS-CoV-2 spike proteins in phosphate-buffered saline (PBS) and artificial saliva buffers to mimic real human SARS-CoV-2 samples, covering a wide range of concentrations. The sensor exhibits a detection limit of 0.2 ng mL (1.5 pM) for spike proteins. Middle East respiratory syndrome (MERS-CoV) spike proteins are differentiated from SARS-CoV-2 spike proteins, demonstrating specificity.
这项工作探索了无标记生物传感作为一种有效的生物分子分析方法，以确保天然构象和生物活性的保留。重点是一种利用基于微纳加工纳米孔的阻抗传感器的新型电子生物传感平台，为严重急性呼吸综合征冠状病毒2（SARS-CoV-2，即新冠病毒）检测提供快速的即时诊断。该纳米孔传感器通过包括沉积、图案化和蚀刻在内的一系列微加工工艺构建在二氧化硅基板上，具有一个用抗体功能化的5×5孔阵列。纳米孔阵列内的实时阻抗变化使得使用少量样本（<5 μL）在十分钟内即可获得诊断结果。该研究包括在磷酸盐缓冲盐水（PBS）和人工唾液缓冲液中对SARS-CoV-2刺突蛋白进行检测，以模拟真实的人类SARS-CoV-2样本，涵盖了广泛的浓度范围。该传感器对刺突蛋白的检测限为0.2 ng/mL（1.5 pM）。中东呼吸综合征（MERS-CoV）刺突蛋白与SARS-CoV-2刺突蛋白得以区分，显示出特异性。
