Raji Hassan, Xie Pengfei, Tayyab Muhammad, Meng Zhuolun, Mahmoodi Seyed Reza, Javanmard Mehdi
Department of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ, 08854, USA.
Department of Bioengineering and California Institute for Quantitative Biosciences (QB3), University of California Berkeley, Berkeley, CA, 94720, USA.
Biomed Microdevices. 2025 Jan 10;27(1):2. doi: 10.1007/s10544-024-00728-9.
Wearable and implantable biosensors have rapidly entered the fields of health and biomedicine to diagnose diseases and physiological monitoring. The use of wired medical devices causes surgical complications, which can occur when wires break, become infected, generate electrical noise, and are incompatible with implantable applications. In contrast, wireless power transfer is ideal for biosensing applications since it does not necessitate direct connections between measurement tools and sensing systems, enabling remote use of the biosensors. In addition, wireless sensors eliminate the need for a battery or energy harvester, reducing the size of the sensor. As far as we are aware, this is the first report ever describing a new method for wireless readout of a label-free electronic biosensor for detecting protein biomarkers. Our results reveal that we are able to successfully detect target protein and corresponding antibodies within this wireless setup. We are able to distinguish target protein in purified samples from a blank PBS sample as a negative control by tracking gradual changes in impedance at the input of the transmitter (P-value = 0.00788). We also demonstrate real-time wireless quantification of cytokines within rheumatoid arthritis patient serum samples (P-value = 0.00891). A Fine Gaussian Support Vector Machine is also used to differentiate protein from negative controls with the highest accuracy from a dataset of 54 experiments.
可穿戴和可植入生物传感器已迅速进入健康和生物医学领域，用于疾病诊断和生理监测。有线医疗设备的使用会引发手术并发症，当导线断裂、感染、产生电噪声以及与可植入应用不兼容时，这些并发症就可能出现。相比之下，无线电力传输对于生物传感应用来说是理想之选，因为它无需测量工具和传感系统之间的直接连接，从而能够远程使用生物传感器。此外，无线传感器无需电池或能量收集器，减小了传感器的尺寸。据我们所知，这是有史以来第一篇描述用于检测蛋白质生物标志物的无标记电子生物传感器无线读出新方法的报告。我们的结果表明，在这种无线设置下，我们能够成功检测目标蛋白质和相应抗体。通过跟踪发射器输入端阻抗的逐渐变化，我们能够将纯化样品中的目标蛋白质与作为阴性对照的空白PBS样品区分开来（P值 = 0.00788）。我们还展示了类风湿性关节炎患者血清样品中细胞因子的实时无线定量（P值 = 0.00891）。在54个实验的数据集中，还使用了精细高斯支持向量机以最高准确率区分蛋白质与阴性对照。
