Yamanaka Aléia Harumi Uchibaba, de Alencar Josiane Bazzo, de Souza Victor Hugo, Zacarias Joana Maiara Valentini, Bahls-Pinto Larissa Danielle, Silva Cléverson O, Sell Ana Maria, de Lima Neto Quirino Alves
Department of Clinical Analysis and Biomedicine, Maringá State University, Maringá, Paraná, Brazil.
Department of Basic Chairs, Marília Medical School-FAMEMA, Marília, São Paulo, Brazil.
Int J Dent. 2025 May 2;2025:7599713. doi: 10.1155/ijod/7599713. eCollection 2025.
Periodontitis (PD) is an inflammatory disease that affects the protective and supporting tissues of teeth. Mutations in cytokines and their receptors may influence the immunopathogenesis of PD, but the role of interleukin-33 (IL-33) and IL1RL1 is not clear. Thus, this study aimed to analyze the polymorphisms in (rs1929992 and rs7025417) and (rs11685424 and rs3821204) genes and the IL-33 serum levels in PD patients. A case-control study was performed with 186 PD patients and 189 controls. Genotyping was performed by polymerase chain reaction using sequence-specific primer (PCR-SSP) technique. Serum levels of IL-33 were determined using the immunoenzymatic method. Statistical analyses were performed using SNPStats and OpenEpi. > 0.05 was considered statistically significant. The rs7025417 C/C genotype was a risk factor for PD in nonsmokers (=0.0015) regardless of smoking status and gender. In the general population, the rs3821204 G/G genotype was protective for PD (=0.006), regardless of gender. Nevertheless, the IL-33 serum levels were increased in patients compared to controls ( < 0.0001); however, no difference was observed among PD patients. The polymorphisms rs7025417 and rs3821204 were associated with risk and protection, respectively, and the production of IL-33 was higher in PD patients than in controls, independent of the extent or severity of the disease.
牙周炎（PD）是一种影响牙齿保护和支持组织的炎症性疾病。细胞因子及其受体的突变可能会影响牙周炎的免疫发病机制，但白细胞介素-33（IL-33）和IL1RL1的作用尚不清楚。因此，本研究旨在分析IL1RL1基因（rs1929992和rs7025417）和IL33基因（rs11685424和rs3821204）的多态性以及牙周炎患者的IL-33血清水平。对186例牙周炎患者和189例对照进行了病例对照研究。采用序列特异性引物聚合酶链反应（PCR-SSP）技术进行基因分型。使用免疫酶法测定IL-33的血清水平。使用SNPStats和OpenEpi进行统计分析。P>0.05被认为具有统计学意义。无论吸烟状况和性别如何，IL1RL1基因的rs7025417 C/C基因型是非吸烟者患牙周炎的危险因素（P=0.0015）。在普通人群中，无论性别如何，IL33基因的rs3821204 G/G基因型对牙周炎具有保护作用（P=0.006）。然而，与对照组相比，患者的IL-33血清水平升高（P<0.0001）；然而，在牙周炎患者中未观察到差异。IL1RL1基因的rs7025417和IL33基因的rs3821204多态性分别与风险和保护相关，并且牙周炎患者中IL-33的产生高于对照组，与疾病的范围或严重程度无关。
