Carey Lauren, Malka Osnat, Morin Shai, Robin Charles
University of Melbourne, Melbourne, Victoria, Australia.
Hebrew University of Jerusalem, Israel.
MicroPubl Biol. 2025 Apr 24;2025. doi: 10.17912/micropub.biology.001525. eCollection 2025.
Linamarase is a plant β-glucosidase enzyme involved in the activation of plant protoxins. It thereby plays a key role in plant defense mechanisms against herbivory. We have taken the linamarase gene sequence from cassava and placed it into the genome of Drosophila melanogaster under the control of non-leaky heat-shock promoter. We show that Drosophila larvae carrying the transgene become sensitive to the pro-toxin linamarin after heat-shock. Furthermore, the sensitivity is elevated in sealed containers and control-larvae sharing such containers with linamarase-larvae are also sensitive, suggesting that the larvae are dying from poisoning with gaseous hydrogen cyanide.
亚麻苦苷酶是一种植物β-葡萄糖苷酶，参与植物原毒素的激活。因此，它在植物抵御食草动物的防御机制中起着关键作用。我们从木薯中获取了亚麻苦苷酶基因序列，并将其置于非渗漏热休克启动子的控制下，导入黑腹果蝇的基因组中。我们发现，携带转基因的果蝇幼虫在热休克后对原毒素亚麻苦苷变得敏感。此外，在密封容器中这种敏感性会增强，与携带亚麻苦苷酶的幼虫共用此类容器的对照幼虫也很敏感，这表明幼虫死于气态氰化氢中毒。
