Ramalho Douglas Lisboa, Silva Jadyellen Rondon, Monteiro Martins Dos Santos Maria Eduarda, Soares Maria Eduarda Silva, Jesus Andrielle Adelina Teodoro, Alves Thiago Henrique Oliveira, Brugnera Michelle Fernanda, Moura Sidnei, Souza Anderson Oliveira
Mitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato Grosso, Cuiabá, Brazil.
Postgraduate Program in Biodiversity and Biotechnology of the BIONORTE Network (PPG-BIONORTE), Federal University of Mato Grosso, Cuiabá, Brazil.
Sci Rep. 2025 May 12;15(1):16447. doi: 10.1038/s41598-025-86413-0.
Photodegradation is a promising technique for remediating contaminated environmental matrices. It demonstrates significant potential in transforming organic contaminants into carbon dioxide, water, and inorganic anions through degradation reactions that involve transient oxidizing species, mainly hydroxyl radicals generated by ultraviolet (UV) irradiation. In this study, we investigated whether the photodegradation of Paraquat (PQ) with UV irradiation reduced its toxicity in Drosophila melanogaster. Our results indicate that ingesting PQ degradation products by larvae resulted in a low axial ratio (pupal volume). In the adults, it resulted in markedly diminished climbing ability in a time-dependent manner after 10 days of feeding. In addition, exposure of D. melanogaster to photodegradation of PQ reduced acetylcholinesterase and citrate synthase activities but improved oxidative stress, as evidenced by protein carbonyl, and lactate production. These results suggest that the photodegradation of PQ with UV irradiation produced PQ fragments with higher toxicity than PQ, while the precise mechanism of its action requires further investigation.
光降解是一种修复受污染环境基质的很有前景的技术。它在通过涉及瞬态氧化物种（主要是紫外线（UV）照射产生的羟基自由基）的降解反应将有机污染物转化为二氧化碳、水和无机阴离子方面显示出巨大潜力。在本研究中，我们调查了用紫外线照射百草枯（PQ）进行光降解是否会降低其对黑腹果蝇的毒性。我们的结果表明，幼虫摄入PQ降解产物会导致低轴比（蛹体积）。在成虫中，喂食10天后，其攀爬能力会以时间依赖的方式显著下降。此外，黑腹果蝇暴露于PQ的光降解过程中会降低乙酰胆碱酯酶和柠檬酸合酶的活性，但会改善氧化应激，这通过蛋白质羰基和乳酸生成得到证明。这些结果表明，用紫外线照射对PQ进行光降解产生的PQ片段比PQ具有更高的毒性，而其作用的确切机制需要进一步研究。
