Xie Fugui, Sun Yali, Zhang Huilan, Cui Junjie, Wang Qing, Gao Xiquan
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, P. R. China.
College of Agriculture, Nanjing Agricultural University, Nanjing, P. R. China.
Plant Signal Behav. 2025 Dec;20(1):2502739. doi: 10.1080/15592324.2025.2502739. Epub 2025 May 12.
Gibberella stalk rot (GSR) caused by is one of the most devastating diseases of maize, not only seriously affecting its yield and the application of mechanized harvest technology but also producing a variety of toxins, thus seriously impacting the food safety. BAK1 (Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1, BAK1) is the well-studied co-receptor of PRRs (Pattern Recognition Receptors), which is involved in the regulation of growth and development regulation as well as the response to diverse biological stresses. However, little is known about the role of BAK1 in the interaction between maize and pathogens, especially in maize against GSR. In this study, we found that ZmBAK1 () was located at the cytoplasmic membrane. Furthermore, was induced by multiple PAMPs (Pathogen-Associated Molecular Patterns), while PTI (PAMP-Triggered Immunity) response including ROS (Reactive Oxygen Species) burst and callose deposition, as well as cell death, and immune gene expression was weakened in mutant upon PAMP treatment. On the contrary, the ROS production and cell death in -OE were significantly stronger than wild type. Furthermore, mutant is more susceptible to GSR, while -OE is more resistant, compared to wild types. Taken together, our data suggested that ZmBAK1 plays a positive role in maize GSR resistance, likely via activating PTI signaling pathway.
由[病原体名称未给出]引起的赤霉菌茎腐病（GSR）是玉米最具毁灭性的病害之一，不仅严重影响其产量和机械化收获技术的应用，还会产生多种毒素，从而严重影响食品安全。BAK1（油菜素内酯不敏感1相关受体激酶1，BAK1）是研究充分的模式识别受体（PRRs）的共受体，参与生长发育调控以及对多种生物胁迫的响应。然而，关于BAK1在玉米与病原体相互作用中的作用，尤其是在玉米抗GSR方面的作用，人们了解甚少。在本研究中，我们发现ZmBAK1（[基因名称未给出]）定位于细胞质膜。此外，[基因名称未给出]由多种病原体相关分子模式（PAMPs）诱导，而在PAMP处理后，包括活性氧（ROS）爆发和胼胝质沉积以及细胞死亡在内的PAMP触发的免疫（PTI）反应以及免疫基因表达在[突变体名称未给出]突变体中减弱。相反，在[过表达株系名称未给出]-OE中ROS产生和细胞死亡明显强于野生型。此外，与野生型相比，[突变体名称未给出]突变体对GSR更敏感，而[过表达株系名称未给出]-OE更抗病。综上所述，我们的数据表明ZmBAK1可能通过激活PTI信号通路在玉米抗GSR中发挥积极作用。
