Bai Hua, Si Helong, Zang Jinping, Pang Xi, Yu Lu, Cao Hongzhe, Xing Jihong, Zhang Kang, Dong Jingao
State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, China.
College of Life Sciences, Hebei Agricultural University, Baoding, China.
Front Plant Sci. 2021 Aug 13;12:694973. doi: 10.3389/fpls.2021.694973. eCollection 2021.
is the causal agent of stalk rot in maize stem, resulting in maize lodging, yield, quality, and mechanical harvesting capacity. To date, little is known about the maize stem defense mechanism in response to the invasion of . This study represents a global proteomic approach to document the infection by . A total of 1,894 differentially expressed proteins (DEPs) were identified in maize stem with inoculation. Functional categorization analysis indicated that proteins involved in plant-pathogen interaction were inducible at the early stages of infection. We also found that the expression of proteins involved in phenylpropanoid, flavonoid, and terpenoid biosynthesis were upregulated in response to infection, which may reflect that these secondary metabolism pathways were important in the protection against the fungal attack in maize stem. In continuously upregulated proteins after infection, we identified a WRKY transcription factor, ZmWRKY83, which could improve the resistance to plant pathogens. Together, the results show that the defense response of corn stalks against infection was multifaceted, involving the induction of proteins from various immune-related pathways, which had a directive significance for molecular genetic breeding of maize disease-resistant varieties.
是玉米茎腐病的致病因子，导致玉米倒伏、产量、品质和机械收获能力下降。迄今为止，关于玉米茎对入侵的防御机制知之甚少。本研究采用全球蛋白质组学方法记录了的感染情况。在接种的玉米茎中总共鉴定出1894个差异表达蛋白（DEPs）。功能分类分析表明，参与植物-病原体相互作用的蛋白在感染早期被诱导。我们还发现，参与苯丙烷类、黄酮类和萜类生物合成的蛋白的表达在感染后上调，这可能反映出这些次生代谢途径在保护玉米茎免受真菌攻击方面很重要。在感染后持续上调的蛋白中，我们鉴定出一个WRKY转录因子ZmWRKY83，它可以提高对植物病原体的抗性。总之，结果表明玉米秸秆对感染的防御反应是多方面的，涉及多种免疫相关途径蛋白的诱导，这对玉米抗病品种的分子遗传育种具有指导意义。
