Zhang Pengwei, Dong Xianhui, Zeng Yijun, Chen Junkui, Yang Sijia, Yu Peipei, Ye Chunhong, Hung Wei-Lian, Jiang Qiuyue, Zhao Wen, Zeng Zhaozhong, Li Jinjun, Li Li
The Affiliated Hospital of Hangzhou Normal University, Hangzhou Normal University, Hangzhou, 310015, China.
Hangzhou Normal University, Hangzhou, 310015, China.
Probiotics Antimicrob Proteins. 2024 Nov 6. doi: 10.1007/s12602-024-10395-0.
Lacticaseibacillus paracasei K56 (L. paracasei K56) is a probiotic with weight-loss effects. However, symbiosis research on the combined effects of Lacticaseibacillus paracasei K56 and prebiotics is lacking. Therefore, the aim of this study was to investigate the effects of L. paracasei K56, xylooligosaccharide (XOS), galactooligosaccharide (GOS), polyglucose (PG), and their synbiotic combinations (XOS + K56, GOS + K56, and PG + K56) on metabolism and gut composition in children with obesity, using an in vitro fermentation model. Fecal samples were collected from 14 children with obesity for in vitro fermentation, and the effects of the various treatments in gas production and short chain fatty acid synthesis (SCFAs) were assessed. Treatment with probiotics, prebiotics, and synbiotics regulated gut microbiota and metabolites in children with obesity. GOS and XOS had higher degradation rates than PG + K56 synbiotics in the gut microbiota of children with obesity. Moreover, treatment with XOS, GOS, and their synbiotic combinations, (XOS + K56) and (GOS + K56), significantly reduced the production of gas, propionic acid, and butyric acid compared with PG + K56 treatment. Treatments with GOS + K56 and XOS + K56 altered the composition of the gut microbiota, improved the abundance of Bifidobacteria and Lactobacilli, and reduced the abundance of Escherichia/Shigella. Overall, this study provides a theoretical foundation for the use of K56-based synbiotics.
副干酪乳杆菌K56（L. paracasei K56）是一种具有减肥作用的益生菌。然而，关于副干酪乳杆菌K56与益生元联合作用的共生研究尚缺乏。因此，本研究的目的是使用体外发酵模型，研究L. paracasei K56、低聚木糖（XOS）、低聚半乳糖（GOS）、聚葡萄糖（PG）及其合生元组合（XOS + K56、GOS + K56和PG + K56）对肥胖儿童代谢和肠道组成的影响。从14名肥胖儿童中采集粪便样本进行体外发酵，并评估各种处理对气体产生和短链脂肪酸合成（SCFAs）的影响。益生菌、益生元和合生元处理可调节肥胖儿童的肠道微生物群和代谢产物。在肥胖儿童的肠道微生物群中，GOS和XOS的降解率高于PG + K56合生元。此外，与PG + K56处理相比，XOS、GOS及其合生元组合（XOS + K56）和（GOS + K56）处理显著降低了气体、丙酸和丁酸的产生。GOS + K56和XOS + K56处理改变了肠道微生物群的组成，提高了双歧杆菌和乳杆菌的丰度，并降低了埃希氏菌属/志贺氏菌属的丰度。总体而言，本研究为基于K56的合生元的使用提供了理论基础。
