Seow W K, Crouch A A, Thong Y H
Trans R Soc Trop Med Hyg. 1985;79(3):359-62. doi: 10.1016/0035-9203(85)90380-3.
A simple rapid method for the measurement of adherence of Giardia lamblia trophozoites is described. Microcolumns are prepared from 10 mg amounts of nylon fibre packed into 100 microliter size disposable pipette tips. The kinetics show that 30 min is the optimum contact time for the experiments. Maximum adherence occurred at 37 degrees C, minimal at 4 degrees C, and intermediate at 25 degrees C, suggesting that adherence is an energy-dependent process. Adherence was fluoride-sensitive but cyanide-insensitive, suggesting that this parasite derives its energy from the glycolytic pathway, and not the oxidative pathway. Metronidazole, one of the mainstays for chemotherapy of giardiasis, produced significant inhibition of adherence at 10 micrograms/ml and 100 micrograms/ml concentrations after one hour pre-incubation.
本文描述了一种用于测量蓝氏贾第鞭毛虫滋养体黏附性的简单快速方法。将10毫克尼龙纤维装入100微升大小的一次性移液器吸头中制成微柱。动力学研究表明，30分钟是实验的最佳接触时间。最大黏附发生在37摄氏度，最小黏附发生在4摄氏度，25摄氏度时黏附处于中间水平，这表明黏附是一个能量依赖过程。黏附对氟化物敏感但对氰化物不敏感，这表明该寄生虫的能量来源于糖酵解途径而非氧化途径。甲硝唑是治疗贾第虫病的主要药物之一，在预孵育一小时后，10微克/毫升和100微克/毫升浓度的甲硝唑对黏附产生了显著抑制作用。
