Thong Y H, Currell J M
J Immunol Methods. 1983 Oct 14;63(2):229-36. doi: 10.1016/0022-1759(83)90427-1.
The development of a microassay for neutrophil adherence is described. The nylon fibre column consisted of 10 mg of fibre inserted into a micropipette tip. A vacuum harvester was constructed to aspirate the neutrophil-containing fluid in the columns into disposable test-tubes for quantitation of neutrophil concentrations. In this way, the neutrophil requirement was reduced from 10(7) cells to 10(5) cells. A complete assay can be performed efficiently and rapidly using less than 1 ml of blood.
本文描述了一种用于中性粒细胞黏附的微量测定方法的开发。尼龙纤维柱由插入微量移液器吸头的10毫克纤维组成。构建了一个真空收集器，用于将柱中含中性粒细胞的液体吸入一次性试管中，以定量中性粒细胞浓度。通过这种方式，中性粒细胞的需求量从10⁷个细胞减少到了10⁵个细胞。使用不到1毫升的血液即可高效快速地完成完整测定。
