Hessel E, Lerche D
Vox Sang. 1985;49(2):86-91. doi: 10.1111/j.1423-0410.1985.tb00774.x.
Aggregation measurement of washed human erythrocytes (RBC) were carried out in a NaCl-PBS solution under laminar shear conditions. Artificial aggregation of fresh and stored erythrocytes was caused by decreased pH and reduced ionic strength and characterized by collision efficiency alpha. Generally, the collision efficiency alpha of stored erythrocytes rises with the increased storage time. Such an aggregation technique might be useful to detect and quantify changes of the membrane and/or the surface structure due to aging and/or storage.
在层流剪切条件下,于NaCl-PBS溶液中对洗涤过的人红细胞(RBC)进行聚集测量。新鲜和储存红细胞的人工聚集是由pH值降低和离子强度降低引起的,其特征在于碰撞效率α。一般来说,储存红细胞的碰撞效率α会随着储存时间的增加而升高。这种聚集技术可能有助于检测和量化由于老化和/或储存导致的膜和/或表面结构的变化。