Fu Xiaomeng, Hong Kunqiang
College of Food Science and Technology, Wuhan Business University, Wuhan 430056, China.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Foods. 2025 Apr 27;14(9):1533. doi: 10.3390/foods14091533.
cAMP (cyclic adenosine-3',5'-monophosphate) has extensive physiological functions and nutritional value for living organisms, and it regulates cellular metabolism mainly by modulating PKA (protein kinase A) activity. The current yields of cAMP synthesized by microbial fermentation are still low, which is arousing interest in developing high-yield cAMP strains. In this work, two baker's yeasts with high cAMP content were constructed by knocking out , , and , and truncating the promoter of the . The content of cAMP in BN5-126 and BN5-310 (with the gene promoter truncated by 126 and 310 bp in BN5) was improved by 30- and 9-fold, respectively, relative to the wild strain. The gene mRNA levels of BN5-126 and BN5-310 were decreased by 18% and 40%, respectively, without significant changes in growth performance. The results of heat shock tolerance of engineered strains also reflected the enhanced PKA activity. This work demonstrates a novel strategy for regulating gene expression to boost cAMP biosynthesis in yeast, providing a promising platform for producing nutritionally enriched and functional fermented products.
环磷酸腺苷（cAMP，即3',5'-环化腺苷单磷酸）对生物体具有广泛的生理功能和营养价值，它主要通过调节蛋白激酶A（PKA）的活性来调控细胞代谢。目前通过微生物发酵合成的环磷酸腺苷产量仍然较低，这引发了人们对开发高产环磷酸腺苷菌株的兴趣。在这项研究中，通过敲除、和，并截短的启动子，构建了两株环磷酸腺苷含量高的面包酵母。相对于野生菌株，BN5-126和BN5-310（在BN5中基因启动子分别截短了126和310 bp）中的环磷酸腺苷含量分别提高了30倍和9倍。BN5-126和BN5-310的基因mRNA水平分别降低了18%和40%，而生长性能没有显著变化。工程菌株的热休克耐受性结果也反映了PKA活性的增强。这项工作展示了一种调节基因表达以提高酵母中环磷酸腺苷生物合成的新策略，为生产营养丰富的功能性发酵产品提供了一个有前景的平台。
