转录组分析揭示了E类AmMADS - Box基因与百合花瓣畸形的关联

Transcriptome Analysis Reveals Association of E-Class AmMADS-Box Genes with Petal Malformation in L.

作者信息

Yang Dongmei, Chen Yiwen, He Yutong, Song Jiayi, Jiang Ye, Yang Meiyun, Zheng Xingyan, Wang Li, Hu Huizhen

机构信息

Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China.

出版信息

Int J Mol Sci. 2025 May 7;26(9):4450. doi: 10.3390/ijms26094450.

DOI:10.3390/ijms26094450

PMID:40362686

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12072684/

Abstract

Snapdragon () serves as a model system for dissecting floral morphogenesis mechanisms. Petal malformation in impacts ornamental value, but its genetic basis remains poorly understood. We compared transcriptomes of the wild-type (Am11) and a petal-malformed mutant (AmDP2) to identify 2303 differentially expressed genes (DEGs), including E-class MIKC-type MADS-box genes () and (). Weighted gene co-expression network (WGCNA), protein-protein interaction (PPI), qRT-PCR and virus-induced gene silencing (VIGS) analyses revealed interactions between SEP2/SEP3 and C/A/B-class genes (, , ), co-regulated MADS transcription factors (MTFs) AGL15 (AmMADS16), and auxin signaling genes (, ). qRT-PCR validated upregulation of and downregulation of in AmDP2. Our results suggest that E-class MADS-box genes are associated with petal malformation through coordinated interactions with hormonal pathways. These findings provide candidate targets for further functional studies in snapdragon.

摘要

金鱼草作为剖析花形态发生机制的模式系统。金鱼草中的花瓣畸形会影响观赏价值，但其遗传基础仍知之甚少。我们比较了野生型（Am11）和花瓣畸形突变体（AmDP2）的转录组，以鉴定出2303个差异表达基因（DEGs），包括E类MIKC型MADS盒基因（SEP2和SEP3）。加权基因共表达网络（WGCNA）、蛋白质-蛋白质相互作用（PPI）、qRT-PCR和病毒诱导基因沉默（VIGS）分析揭示了SEP2/SEP3与C/A/B类MADS基因（AP3、PI、AG）之间的相互作用、共同调控的MADS转录因子（MTFs）AGL15（AmMADS16）以及生长素信号基因（YUCCA、ARF）。qRT-PCR验证了AmDP2中SEP2的上调和SEP3的下调。我们的结果表明，E类MADS盒基因通过与激素途径的协同相互作用与花瓣畸形相关。这些发现为金鱼草的进一步功能研究提供了候选靶点。