Wróblewska-Kurdyk Anna, Kordan Bożena, Bocianowski Jan, Stec Katarzyna, Gabryś Beata
Department of Botany and Ecology, University of Zielona Góra, Szafrana 1, 65-516 Zielona Góra, Poland.
Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720 Olsztyn, Poland.
Int J Mol Sci. 2025 May 7;26(9):4452. doi: 10.3390/ijms26094452.
Apigenin and luteolin are products of the phenylpropanoid pathway, where apigenin serves as a substrate for the synthesis of luteolin. Apigenin and luteolin are highly bioactive flavones; therefore, in search of prospective biopesticides, the receptiveness of the polyphagous green peach aphid (Sulzer) (Hemiptera: Aphididae) to apigenin and luteolin was studied. The flavones were applied as 0.1% ethanolic solutions to the host plant leaf surface, and aphid probing and feeding activities were monitored using the Electrical Penetration Graph (EPG) technique. The structural difference between apigenin and luteolin, which was the number of hydroxyl groups in the molecule, had an impact on the activity of these flavones. On apigenin-treated plants, the duration of the first probe was three times as short as on the control and five times as short as on the luteolin-treated plants; the duration of the time to the first ingestion phase within the successful probe was shorter than on the control and luteolin-treated plants; the mean duration of xylem sap ingestion bouts and the proportion of xylem phase in all probing activities were the highest; and the duration of salivation before phloem sap ingestion was the longest. Aphids' response to luteolin-treated plants was less distinct as compared to apigenin-treated plants.
芹菜素和木犀草素是苯丙烷类途径的产物，其中芹菜素是合成木犀草素的底物。芹菜素和木犀草素是具有高生物活性的黄酮类化合物；因此，为了寻找潜在的生物农药，研究了多食性桃蚜（Sulzer）（半翅目：蚜科）对芹菜素和木犀草素的感受性。将黄酮类化合物以0.1%的乙醇溶液形式施用于寄主植物叶片表面，并使用电穿透图（EPG）技术监测蚜虫的刺探和取食活动。芹菜素和木犀草素的结构差异，即分子中的羟基数量，对这些黄酮类化合物的活性有影响。在经芹菜素处理的植物上，首次刺探的持续时间是对照植物的三分之一，是经木犀草素处理植物的五分之一；成功刺探后进入首次取食阶段的时间比对照植物和经木犀草素处理的植物短；木质部汁液取食回合的平均持续时间以及所有刺探活动中木质部阶段的比例最高；在取食韧皮部汁液前的唾液分泌持续时间最长。与经芹菜素处理的植物相比，蚜虫对经木犀草素处理的植物的反应不太明显。
