Wang Yawen, Jiang Yanbing, Liu Ximeng, Chen Yadi, Zhang Qingxia, Wang Li, Li Weixing
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225000, China.
College of Plant Protection, Yangzhou University, Yangzhou 225000, China.
Plants (Basel). 2024 Aug 1;13(15):2122. doi: 10.3390/plants13152122.
is abundant in secondary metabolites, including flavonoids and terpenoids. While the majority of research has focused on the role of these compounds in disease resistance, their specific contribution to pathogen defense has been rarely explored. In this study, we collected root exudates from hydroponically cultivated ginkgo seedlings and conducted a metabolomic analysis. We identified several primary metabolites mainly comprising amino acids and nucleotides, while secondary metabolites consisted of various compounds, including bioactive compounds such as flavonoids and terpenoids. Focusing on the secondary metabolites with relatively higher abundance in the exudates, we selected a mixture of flavonoids and terpenoids for in vitro inhibition experiments against two soil-borne fungal pathogens, f. sp. that causes cucumber wilt and AG-8 that causes wheat root rot. The results indicated that the growth rate of both fungus cells was significantly reduced with the increasing concentration of the flavonoid and terpenoid mixture extracted from ginkgo and was completely inhibited at a concentration of 5 mg/mL. Further experiments revealed that this mixture of flavonoids and terpenoids had a destructive effect on the cellular structure of both fungi, thereby reducing cell viability and achieving an antifungal effect. These findings provide a foundation for further research into the use of ginkgo extracts in biological control.
富含次生代谢产物，包括黄酮类化合物和萜类化合物。虽然大多数研究集中在这些化合物在抗病性中的作用，但它们对病原体防御的具体贡献很少被探索。在本研究中，我们收集了水培银杏幼苗的根系分泌物并进行了代谢组学分析。我们鉴定出几种主要由氨基酸和核苷酸组成的初级代谢产物，而次生代谢产物则由各种化合物组成，包括黄酮类化合物和萜类化合物等生物活性化合物。针对分泌物中相对丰度较高的次生代谢产物，我们选择了黄酮类化合物和萜类化合物的混合物，对两种土传真菌病原体进行体外抑制实验，即引起黄瓜枯萎病的尖孢镰刀菌和引起小麦根腐病的AG-8。结果表明，随着从银杏中提取的黄酮类化合物和萜类化合物混合物浓度的增加，两种真菌细胞的生长速率均显著降低，在浓度为5mg/mL时完全被抑制。进一步的实验表明，这种黄酮类化合物和萜类化合物的混合物对两种真菌的细胞结构都有破坏作用，从而降低细胞活力并实现抗真菌效果。这些发现为进一步研究银杏提取物在生物防治中的应用提供了基础。
