Keem Min-Ji, Kim Tae-Young, Park No-June, Choi Sangho, Paik Jin-Hyub, Jo Beom-Geun, Kwon Taek-Hwan, Kim Su-Nam, Lee Seoung Rak, Yang Min Hye
Department of Pharmacy, Research Institute for Drug Development, College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea.
Nutrients. 2025 Apr 30;17(9):1552. doi: 10.3390/nu17091552.
The genus has been traditionally used in Asia to treat various ailments, including parotitis, pneumonia, and pertussis. These plants contain many bioactive compounds, including flavonoids, coumarins, and lignans. This study investigates the chemical components of a MeOH extract of the aerial parts of (Thunb.) Makino and evaluates their anti-inflammatory and anti-allergic effects in vitro. Chromatographic techniques, spectroscopic analysis, and the literature were used to isolate compounds from the branches and leaves of . IL-4 mRNA and -hexosaminidase levels were assessed by performing assays on RBL-2H3 cells to evaluate anti-inflammatory and anti-allergic potential. Forty-two compounds were isolated from the extract, and the flavanones trichotocinol A and B were newly identified. Screening of isolated compounds showed that several significantly inhibited DNP-BSA-induced -hexosaminidase release by 10.0-58.0% and PMA/ionomycin-induced IL-4 mRNA expression by 25.3-71.7% versus negative controls. In addition, trichotocinol A reduced IL-4 mRNA expression by 31.9%. The discovery of these new compounds contributes to our understanding of the bioactive properties of and suggests their potential use as natural therapeutic agents for inflammatory disorders.
该属植物在亚洲传统上用于治疗各种疾病，包括腮腺炎、肺炎和百日咳。这些植物含有许多生物活性化合物，包括黄酮类、香豆素类和木脂素类。本研究调查了（Thunb.）Makino地上部分甲醇提取物的化学成分，并评估了它们在体外的抗炎和抗过敏作用。采用色谱技术、光谱分析和文献资料从的枝叶中分离化合物。通过对RBL-2H3细胞进行检测来评估白细胞介素-4信使核糖核酸（IL-4 mRNA）水平和β-己糖胺酶水平，以评价其抗炎和抗过敏潜力。从提取物中分离出42种化合物，新鉴定出黄烷酮类化合物毛钩藤醇A和B。对分离出的化合物进行筛选表明，与阴性对照相比，几种化合物显著抑制了二硝基苯基牛血清白蛋白（DNP-BSA）诱导的β-己糖胺酶释放10.0 - 58.0%，以及佛波酯（PMA）/离子霉素诱导的IL-4 mRNA表达25.3 - 71.7%。此外，毛钩藤醇A使IL-4 mRNA表达降低了31.9%。这些新化合物的发现有助于我们了解的生物活性特性，并表明它们作为炎症性疾病天然治疗剂的潜在用途。
