Oruc Muhammed Enes, Evcimen Duygulu Nilüfer, Onder Betul, Yelkenci Aslihan, Ustündag Cem Bülent, Ciftci Fatih
Department of Chemical Engineering, University of Doha for Science and Technology, Doha 24449, Qatar.
Department of Metallurgical and Material Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkey.
Polymers (Basel). 2025 Apr 23;17(9):1148. doi: 10.3390/polym17091148.
In this study, poly(methyl methacrylate) (PMMA) nanofiber scaffolds reinforced with synthesized nano-hydroxyapatite (n-HA) were fabricated through electrospinning to enhance their potential for applications in bone tissue engineering. Sodium tripolyphosphate (STTP) was utilized as a surfactant to achieve a uniform distribution of particles and improve the structural integrity of the scaffolds. PMMA solutions were prepared at concentrations of the addition of STTP effectively stabilized n-HA dispersion, leading to enhanced fiber morphology, as confirmed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). The PMMA_10_HA_S nanofibers demonstrated a homogeneous fiber distribution with an average diameter of 345.40 ± 53.55 nm and a calcium content of 7.1%. Mechanical testing revealed that adding STTP enhanced the mechanical properties, with the n-HA-reinforced 10 wt.% PMMA nanofibers achieving a maximum tensile stress of 4.16 ± 2.13 MPa and an elongation of 7.1 ± 1.95%. Furthermore, cell cytotoxicity assays of different concentrations (25, 50, 75, and 100 mg/mL) using L929 fibroblast cells demonstrated no cytotoxic effect of PMMA_10_HA_S nanofibers. These findings, reinforced by STTP and n-HA, highlight the potential of PMMA_10_HA_S nanofiber scaffolds as promising candidates for bone tissue applications.
在本研究中，通过静电纺丝制备了用合成纳米羟基磷灰石（n-HA）增强的聚甲基丙烯酸甲酯（PMMA）纳米纤维支架，以提高其在骨组织工程中的应用潜力。使用三聚磷酸钠（STTP）作为表面活性剂，以实现颗粒的均匀分布并改善支架的结构完整性。制备了浓度为添加STTP有效稳定n-HA分散体的PMMA溶液，从而改善了纤维形态，扫描电子显微镜（SEM）、能量色散光谱（EDS）和透射电子显微镜（TEM）证实了这一点。PMMA_10_HA_S纳米纤维显示出均匀的纤维分布，平均直径为345.40±53.55 nm，钙含量为7.1%。力学测试表明，添加STTP增强了力学性能，n-HA增强的10 wt.%PMMA纳米纤维的最大拉伸应力为4.16±2.13 MPa，伸长率为7.1±1.95%。此外，使用L929成纤维细胞对不同浓度（25、50、75和100 mg/mL）进行的细胞毒性测定表明，PMMA_10_HA_S纳米纤维没有细胞毒性作用。这些由STTP和n-HA强化的发现突出了PMMA_10_HA_S纳米纤维支架作为骨组织应用有前景的候选材料的潜力。
