Department of Polymer Science and Engineering , Kyungpook National University, Daegu, South Korea.
J Biomater Sci Polym Ed. 2013;24(1):61-76. doi: 10.1163/156856212X623526. Epub 2012 May 11.
Hydroxyapatite (HA)-containing polymers have been proposed for improving the biological properties of bone cements. Poly(methyl methacrylate) (PMMA) has long been used to secure orthopedic implants to skeletal bones. The aim of this study was to determine whether the incorporation of HA nanoparticles into the PMMA nanofibrous scaffolds enhances the biological functions of osteoblasts. The number of osteoblasts adhered and proliferated on the PMMA/HA nanofibrous scaffolds was significantly larger than that on the PMMA alone. The cytoskeletal organization and alkaline phosphatase (ALP) activity of the osteoblasts on the PMMA/HA nanofibrous scaffolds were clearly higher than that on the PMMA control. The amount of calcium ions released from 20 wt% HA-containing PMMA nanofibrous scaffolds (PMMA/HA20) was much higher than that released from 10 wt% HA-containing PMMA nanofibrous scaffolds (PMMA/HA10) (HA, 10 wt%). These findings suggested that osteoblast differentiation was accelerated by the incorporation of HA into the PMMA nanofibrous scaffolds. Therefore, the incorporation of HA into the PMMA nanofibrous scaffolds could be a useful method. This can be used for providing PMMA scaffolds with enhanced osteogenic properties.
含羟磷灰石（HA）的聚合物已被提议用于改善骨水泥的生物学性能。聚甲基丙烯酸甲酯（PMMA）长期以来一直用于将骨科植入物固定在骨骼上。本研究旨在确定将 HA 纳米粒子掺入 PMMA 纳米纤维支架中是否会增强成骨细胞的生物学功能。与单独的 PMMA 相比，HA 纳米粒子掺入 PMMA 纳米纤维支架上的成骨细胞的粘附和增殖数量明显更多。PMMA/HA 纳米纤维支架上成骨细胞的细胞骨架组织和碱性磷酸酶（ALP）活性明显高于 PMMA 对照。从 20wt%HA 含量的 PMMA 纳米纤维支架（PMMA/HA20）中释放的钙离子量明显高于从 10wt%HA 含量的 PMMA 纳米纤维支架（PMMA/HA10）（HA，10wt%）。这些发现表明，HA 的掺入加速了 PMMA 纳米纤维支架中成骨细胞的分化。因此，将 HA 掺入 PMMA 纳米纤维支架中可能是一种有用的方法。这可用于为 PMMA 支架提供增强的成骨特性。
