Wongtechanon Suprawee, Noosak Chayanee, Jantorn Pavarish, Watcharanurak Papitchaya, Swangphon Piyawut, Wanna Warapond, Sotthibandhu Dennapa Saeloh
Faculty of Medical Technology, Prince of Songkla University, Songkhla 90110, Thailand.
Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
Polymers (Basel). 2025 May 3;17(9):1246. doi: 10.3390/polym17091246.
The development of effective wound dressings remains a critical challenge in medical treatments, requiring materials that promote healing, minimize infection, and enhance tissue regeneration. This study evaluated the wound-healing potential of sericin-based film-forming gels. Six formulations were developed by combining varying concentrations of sericin, a protein derived from silk cocoons, with polyvinyl alcohol (PVA). These formulations were evaluated for physical properties including drying time, pH, spreadability, stability, swelling ratio, flexibility, and adhesion. Film-forming gel is an attractive option for wound dressing due to its flexibility, adhesion, and infrequent reapplication. The F4 formulation (1% sericin) demonstrated superior performances in drying time, spreadability, stability, swelling ratio, flexibility, and skin adhesion, was easy to apply, and formed a stable film on drying. Biological evaluations showed that F4 exhibited excellent compatibility with skin fibroblast cells, maintained a suitable pH, and significantly promoted cell proliferation and migration. The F4 formulation also demonstrated anti-inflammatory effects by inhibiting iNOS expression and nitric oxide production, offering mechanical stability, biological activity, and ease of use with significant potential for treating acute and chronic wounds.
开发有效的伤口敷料仍然是医学治疗中的一项关键挑战，需要能够促进愈合、减少感染并增强组织再生的材料。本研究评估了基于丝胶蛋白的成膜凝胶的伤口愈合潜力。通过将不同浓度的丝胶蛋白（一种源自蚕茧的蛋白质）与聚乙烯醇（PVA）混合，开发了六种配方。对这些配方进行了物理性能评估，包括干燥时间、pH值、铺展性、稳定性、溶胀率、柔韧性和粘附性。成膜凝胶因其柔韧性、粘附性和较少的重新涂抹次数而成为伤口敷料的一个有吸引力的选择。F4配方（1%丝胶蛋白）在干燥时间、铺展性、稳定性、溶胀率、柔韧性和皮肤粘附性方面表现出优异的性能，易于应用，干燥后形成稳定的薄膜。生物学评估表明，F4与皮肤成纤维细胞具有出色的相容性，保持合适的pH值，并显著促进细胞增殖和迁移。F4配方还通过抑制诱导型一氧化氮合酶（iNOS）表达和一氧化氮产生表现出抗炎作用，具有机械稳定性、生物活性且易于使用，在治疗急性和慢性伤口方面具有巨大潜力。
