Kołodziejczyk Agnieszka Maria, Grala Magdalena, Kołodziejczyk Łukasz
Nanomaterial Structural Research Laboratory and Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Dubois 114/116, 93-465 Lodz, Poland.
Food Science Department, Faculty of Pharmacy, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
Molecules. 2025 May 2;30(9):2024. doi: 10.3390/molecules30092024.
Gold nanoparticles stabilized with polyamidoamine dendrimers are one of the potential candidates for use as a contrast agent in computed tomography and a drug delivery agent. This work demonstrates a rapid, two-step synthesis of such complexes, which are size-stable for up to 18 months. The first step of the synthesis involves a short sonication of gold (III) chloride hydrate with polyamidoamine dendrimers of the fourth generation, while the second step uses microwaves to reduce gold (III) chloride hydrate with sodium citrate. The developed synthesis method enables rapid production of spherical and monodisperse gold nanoparticles stabilized with polyamidoamine dendrimers. Physicochemical characterization of the gold nanoparticle-polyamidoamine dendrimers complexes was performed using ultraviolet-visible spectroscopy, dynamic light scattering technique, infrared spectroscopy, atomic force microscopy, and transmission electron microscopy. The toxicity of synthesized complexes on the breast cancer MCF-7 cell line has been studied using the tetrazolium salt reduction test. The produced gold nanoparticles revealed lower toxicity levels on the MCF-7 cell line after 18 months from synthesis compared with newly synthesized colloids. Synthesized gold nanoparticles stabilized with dendrimers and commercially available gold nanoparticles stabilized with sodium citrate show similar toxicity levels on breast cancer cells.
用聚酰胺胺树枝状大分子稳定的金纳米颗粒是用作计算机断层扫描造影剂和药物递送剂的潜在候选物之一。这项工作展示了此类复合物的快速两步合成法，该复合物在长达18个月的时间内尺寸稳定。合成的第一步涉及将水合氯化金(III)与第四代聚酰胺胺树枝状大分子进行短时间超声处理，而第二步使用微波用柠檬酸钠还原水合氯化金(III)。所开发的合成方法能够快速生产用聚酰胺胺树枝状大分子稳定的球形且单分散的金纳米颗粒。使用紫外可见光谱、动态光散射技术、红外光谱、原子力显微镜和透射电子显微镜对金纳米颗粒-聚酰胺胺树枝状大分子复合物进行了物理化学表征。使用四唑盐还原试验研究了合成复合物对乳腺癌MCF-7细胞系的毒性。与新合成的胶体相比，合成后18个月的金纳米颗粒对MCF-7细胞系显示出较低的毒性水平。用树枝状大分子稳定的合成金纳米颗粒和用柠檬酸钠稳定的市售金纳米颗粒对乳腺癌细胞显示出相似的毒性水平。
