Grala Magdalena, Kołodziejczyk Agnieszka M, Białkowska Kamila, Walkowiak Bogdan, Komorowski Piotr
Nanomaterial Structural Research Laboratory, Bionanopark Ltd, Lodz, Poland; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd, Lodz, Poland.
Nanomaterial Structural Research Laboratory, Bionanopark Ltd, Lodz, Poland; Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd, Lodz, Poland.
Micron. 2023 May;168:103430. doi: 10.1016/j.micron.2023.103430. Epub 2023 Feb 27.
Civilization diseases, cancer, frequent mutations of viruses and other pathogens constitute the need to look for new drugs, as well as systems for their targeted delivery. One of the promising way of using drugs is supplying them by linking to nanostructures. One of the solution for the development of nanobiomedicine are metallic nanoparticles stabilized with various polymer structures. In this report, we present the synthesis of gold nanoparticles, their stabilization with polyamidoamine (PAMAM) dendrimers with ethylenediamine core and the characteristics of the obtained product (AuNPs/PAMAM). The presence, size and morphology of synthesized gold nanoparticles were evaluated by ultraviolet-visible light spectroscopy, transmission electron microscopy and atomic force microscopy. The hydrodynamic radius distribution of the colloids was analyzed by dynamic light scattering technique. Additionally, the cytotoxicity and changes in mechanical properties of human umbilical vein endothelial cell line (HUVEC) cells caused by AuNPs/PAMAM were assessed. The results of studies on the nanomechanical properties of cells suggest a two-step changes in cell elasticity as a response to contact with nanoparticles. When using AuNPs/PAMAM in lower concentrations, no changes in cell viability were observed and the cells were softer than untreated cells. When higher concentrations were used, a decrease in the cells viability to about 80 % were observed, as well as non-physiological stiffening of the cells. The presented results may play a significant role in the development of nanomedicine.
文明病、癌症、病毒和其他病原体的频繁变异促使人们寻找新药物以及药物靶向递送系统。使用药物的一种有前景的方法是将药物与纳米结构相连来给药。纳米生物医学发展的一种解决方案是用各种聚合物结构稳定化的金属纳米颗粒。在本报告中，我们展示了金纳米颗粒的合成、用乙二胺核心的聚酰胺胺（PAMAM）树枝状大分子对其进行稳定化以及所得产物（AuNPs/PAMAM）的特性。通过紫外 - 可见光谱、透射电子显微镜和原子力显微镜评估合成金纳米颗粒的存在、尺寸和形态。通过动态光散射技术分析胶体的流体动力学半径分布。此外，评估了AuNPs/PAMAM对人脐静脉内皮细胞系（HUVEC）细胞的细胞毒性和机械性能变化。细胞纳米力学性能的研究结果表明，细胞弹性会因与纳米颗粒接触而出现两步变化。当使用较低浓度的AuNPs/PAMAM时，未观察到细胞活力变化，且细胞比未处理的细胞更柔软。当使用较高浓度时，观察到细胞活力下降至约80%，以及细胞出现非生理性硬化。所呈现的结果可能在纳米医学发展中发挥重要作用。
