Mimi Carolina Ovile, De-la-Cruz-Chacón Iván, da Silva Felipe Moura Araujo, Roberto Victor Cauan Rocha, Ferreira Gisela
Department of Biodiversity and Biostatistics, Institute of Biosciences, São Paulo State University (UNESP), Prof. Dr. Antônio Celso Wagner Zanin Street, 250, Botucatu 18618-689, SP, Brazil.
Laboratorio de Fisiología y Química Vegetal, Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, Libramiento Norte-Poniente 1150, Tuxtla Gutiérrez 29039, CS, Mexico.
Molecules. 2025 May 7;30(9):2070. doi: 10.3390/molecules30092070.
Plant regulators, such as auxins, modulate the synthesis of specialized metabolites and aid in the bioprospection of molecules. is known to produce antifungal alkaloids and serves as a rootstock for . This study evaluated the effects of indoleacetic acid (IAA), indolebutyric acid (IBA), and naphthaleneacetic acid (NAA) applications on the accumulation of alkaloids in ungrafted and grafted with . Total alkaloids were analyzed by spectrophotometry, and alkaloid profiles were analyzed by DI-MS at 8, 14, and 20 days after treatments (DAT). The results indicated that IAA and NAA had the strongest effects on increasing the synthesis of alkaloids in the roots of ungrafted seedlings. In grafted plants, IBA had a more pronounced effect on roots; however, at final evaluation, all three auxins had an impact on both roots and leaves. Chemometric analysis revealed that auxins also altered the alkaloid composition in both seedling types. Nineteen alkaloids were identified regardless of treatment and harvest time. Eight alkaloids were identified for the first time in and nine were identified in . The main alkaloids found in ungrafted seedlings treated with IAA, IBA, and NAA were liriodenine and lanuginosine. In grafted seedlings, liriodenine and reticuline were the primary alkaloids found in roots, whereas liriodenine, lanuginosine, and reticuline were significantly present in leaves. The use of auxins to enhance alkaloid biosynthesis demonstrates their potential for bioprospection and the development of crops tolerant to biotic stress.
植物调节剂，如生长素，可调节特殊代谢产物的合成，并有助于分子的生物勘探。已知[植物名称1]能产生抗真菌生物碱，并作为[植物名称2]的砧木。本研究评估了吲哚乙酸（IAA）、吲哚丁酸（IBA）和萘乙酸（NAA）处理对未嫁接的[植物名称1]以及与[植物名称2]嫁接的[植物名称1]中生物碱积累的影响。在处理后8、14和20天（DAT），通过分光光度法分析总生物碱，并通过直接注入-质谱法（DI-MS）分析生物碱谱。结果表明，IAA和NAA对增加未嫁接幼苗根中生物碱的合成具有最强的作用。在嫁接植物中，IBA对根的影响更为显著；然而，在最终评估时，所有三种生长素对根和叶均有影响。化学计量分析表明，生长素也改变了两种幼苗类型中的生物碱组成。无论处理和收获时间如何，均鉴定出19种生物碱。在[植物名称1]中首次鉴定出8种生物碱，在[植物名称2]中鉴定出9种生物碱。在用IAA、IBA和NAA处理的未嫁接幼苗中发现的主要生物碱是鹅掌楸碱和棉毛苷。在嫁接幼苗中，鹅掌楸碱和网叶番荔枝碱是根中发现的主要生物碱，而鹅掌楸碱、棉毛苷和网叶番荔枝碱在叶中显著存在。使用生长素增强生物碱生物合成证明了它们在生物勘探和培育耐生物胁迫作物方面的潜力。
