Martins Allex Sandro Durão, Huarancca Reyes Thais, Guglielminetti Lorenzo, Damiani Cláudia Roberta
Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79804-970, MS, Brazil.
Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy.
Plants (Basel). 2025 Apr 28;14(9):1331. doi: 10.3390/plants14091331.
Zinc (Zn: 0-400 mg L zinc acetate), lead (Pb: 0-400 mg L lead acetate), and cadmium (Cd: 0-8 mg L cadmium chloride) tolerance in stem explants of Mart. from seeds collected in the Brazilian Cerrado were studied under controlled conditions. The explants showed a regular growth activity in a metal-free medium. All metals did not affect explant survival, except for 400 mg L Zn, which resulted in lethality. Shoot number was not affected by metal treatment, while shoot length and leaf number varied depending on the metal. Cd induced a gradual reduction in leaf number without affecting shoot length. Pb gradually reduced the shoot length at concentrations beyond 200 mg L, while no effects were found in Zn concentrations from 0 to 200 mg L. Pb and Zn induced leaf production at 50 mg L, while a gradual reduction was observed with increasing concentration. Callus formation was not affected by Cd, while increasing Zn and Pb concentrations reduced this cell division and organization with Zn, showing drastic effects. Altogether, explants demonstrated high tolerance to Cd and Pb. However, further studies are needed to explore the phytoextraction capacity of this species at in vitro and planta levels.
对采自巴西塞拉多地区种子的马特氏植物茎外植体，在可控条件下研究了其对锌（Zn：0 - 400毫克/升乙酸锌）、铅（Pb：0 - 400毫克/升乙酸铅）和镉（Cd：0 - 8毫克/升氯化镉）的耐受性。外植体在无金属培养基中表现出正常的生长活性。除400毫克/升的锌导致外植体死亡外，所有金属均未影响外植体的存活。金属处理对外植体的芽数没有影响，但芽长和叶片数因金属而异。镉使叶片数逐渐减少，但不影响芽长。铅在浓度超过200毫克/升时逐渐降低芽长，而在0至200毫克/升的锌浓度下未发现影响。铅和锌在50毫克/升时诱导叶片产生，但随着浓度增加观察到逐渐减少。镉不影响愈伤组织形成，而锌和铅浓度增加会减少这种细胞分裂和组织，锌表现出显著影响。总体而言，外植体对镉和铅表现出高耐受性。然而，需要进一步研究来探索该物种在体外和植株水平的植物提取能力。
