Liu Hunan, Wang Lanjun, Dong Zikun, Wen Shengfang, Liu Changrui, Wang Junpu, Wang Jun, Zhu Lusheng, Kim Young Mo, Wang Jinhua
National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian, 271018, China.
Department of Civil and Environmental Engineering, Hanyang University, Seongdong-gu, Seoul, 04763, Republic of Korea.
Environ Pollut. 2025 Jul 15;377:126444. doi: 10.1016/j.envpol.2025.126444. Epub 2025 May 13.
The application of livestock manure serves as a significant source of ARGs in soils. To study the impact of manure application on the migration of ARGs in the soil-plant system, we set different application ratios (1 %, 3 %, 8 %) of chicken and cow manure for treatment. The study's results demonstrated that the application of organic fertilizers increased the quantity of resistance genes in soil, root, and leaf zones. This change was influenced by the type and proportion of the organic fertilizers used. ARGs and MGEs exhibited the highest absolute enrichment levels in leaf tissues (2.53 and 2.01 times, respectively) with the 3 % cow manure treatment. In contrast, chicken manure exhibited the highest enrichment levels after the 1 % addition (2.51 and 1.81 times, respectively). The applied manure exhibited a high degree of similarity in bacterial community composition with the soil-lettuce system, indicating that ARGs may spread through microorganisms in this system. This study demonstrated that the evolution of bacterial community structure plays a pivotal role in mediating and driving the migration of ARGs within manure-amended soil-plant ecosystems, providing a theoretical basis for understanding the transmission of ARGs in soils and plants due to agricultural production activities.
畜禽粪便的施用是土壤中抗生素抗性基因（ARGs）的重要来源。为研究粪便施用对ARGs在土壤-植物系统中迁移的影响，我们设置了不同施用比例（1%、3%、8%）的鸡粪和牛粪进行处理。研究结果表明，施用有机肥增加了土壤、根区和叶区抗性基因的数量。这种变化受所用有机肥类型和比例的影响。在3%牛粪处理下，ARGs和可移动遗传元件（MGEs）在叶组织中的绝对富集水平最高（分别为2.53倍和2.01倍）。相比之下，鸡粪在添加1%后富集水平最高（分别为2.51倍和1.81倍）。施用的粪便与土壤-生菜系统的细菌群落组成具有高度相似性，表明ARGs可能通过该系统中的微生物传播。本研究表明，细菌群落结构的演变在介导和驱动ARGs在施粪土壤-植物生态系统中的迁移中起关键作用，为理解农业生产活动导致的ARGs在土壤和植物中的传播提供了理论依据。
