Saito Reiko, Yamamoto Yukiyo, Fukano Reiji, Mori Masatomo, Maruyama Takashi, Ueta Yoichi
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan; Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
Peptides. 2025 Jul;189:171409. doi: 10.1016/j.peptides.2025.171409. Epub 2025 May 12.
Acute/chronic exposure to nicotine modulates feeding behavior in experimental animals and humans. However, how nicotine modulates food intake remains unclear. This study examined the acute effects of the peripheral administration of nicotine on food intake in adult male rats, focusing on the possible involvement of the anorectic peptide nesfatin-1/nucleobindin-2 (NucB2) in the central nervous system (CNS). Initially, cumulative food intake, but not water intake, was significantly decreased 0.5, 1, and 1.5 h after the intraperitoneal administration of nicotine (0.5 mg/kg) in 24h-fasted rats. Subsequently, the double-labeled immunohistochemical study revealed that nesfatin-1/NucB2-immunoreactive (ir) neurons expressed Fos-ir in various nuclei of the hypothalamic and brainstem areas, including the supraoptic nucleus and ventral tegmental areas, 90 min after the intraperitoneal administration of nicotine. Finally, pretreatment with intracerebroventricular administration of antisense RNA against nesfatin-1/NucB2 significantly attenuated the suppression of food intake induced by intraperitoneal nicotine administration. The results indicated that the acute effects of peripherally administered nicotine on the suppression of food intake may be partially involved in nesfatin-1/NucB2-containing neurons in the CNS in male adult rats.
急性/慢性接触尼古丁会调节实验动物和人类的进食行为。然而，尼古丁如何调节食物摄入量仍不清楚。本研究考察了成年雄性大鼠外周给予尼古丁对食物摄入量的急性影响，重点关注厌食肽nesfatin-1/核结合蛋白-2（NucB2）在中枢神经系统（CNS）中可能的作用。最初，在禁食24小时的大鼠腹腔注射尼古丁（0.5mg/kg）后0.5、1和1.5小时，累积食物摄入量显著下降，但饮水量未下降。随后，双标免疫组化研究显示，腹腔注射尼古丁90分钟后，nesfatin-1/NucB2免疫反应性（ir）神经元在下丘脑和脑干区域的各个核团中表达Fos-ir，包括视上核和腹侧被盖区。最后，脑室内注射针对nesfatin-1/NucB2的反义RNA预处理显著减弱了腹腔注射尼古丁诱导的食物摄入量抑制。结果表明，外周给予尼古丁对食物摄入量抑制的急性影响可能部分涉及成年雄性大鼠中枢神经系统中含nesfatin-1/NucB2的神经元。
