Takii Ami, Tanabe Yukika, Li Wenting, Shiomi Hiroki, Inoue Akane, Muramatsu Fumitaka, Jia Weizhen, Takakura Nobuyuki
Department of Signal Transduction, Research Institute for Microbial Diseases, The University of Osaka, Suita, Osaka, Japan.
World Premier Institute Immunology Frontier Research Center, The University of Osaka, Osaka, Japan.
Inflamm Regen. 2025 May 14;45(1):14. doi: 10.1186/s41232-025-00379-0.
We previously reported that a vascular endothelial stem cell population resides in pre-existing blood vessels in mice and may contribute to vascular endothelial cells in liver injury or hind limb ischemia models in the long-term. However, whether such stem cells exist in humans and can differentiate specifically into vascular endothelial cells have not been determined. We hypothesized that CD157 vascular endothelial cells in humans may also possess high angiogenic potential.
First, human-derived induced pluripotent stem cells were differentiated into vascular endothelial cells and the expression of CD157 was monitored during the differentiation process. We found that CD157 emerged 11 days after the induction of differentiation, peaked at 14 days, and then declined by 24 days. We also evaluated blood vessel formation by 14- and 24-day-old vascular endothelial cells.
It was found that 14-day-old cells, when CD157 expression was at its peak, formed more blood vessels than 24-day-old cells.
These results suggest that vascular endothelial cells expressing CD157 have high angiogenic potential and may exist as vascular endothelial stem cells.
我们之前报道过，小鼠体内存在一群血管内皮干细胞，它们存在于已有的血管中，并且在长期的肝损伤或后肢缺血模型中可能有助于血管内皮细胞的生成。然而，此类干细胞在人类体内是否存在以及能否特异性分化为血管内皮细胞尚未确定。我们推测人类的CD157血管内皮细胞可能也具有高血管生成潜力。
首先，将人源诱导多能干细胞分化为血管内皮细胞，并在分化过程中监测CD157的表达。我们发现CD157在诱导分化11天后出现，14天时达到峰值，然后在24天时下降。我们还评估了14天龄和24天龄血管内皮细胞的血管形成情况。
发现当CD157表达达到峰值时，14天龄的细胞比24天龄的细胞形成更多的血管。
这些结果表明，表达CD157的血管内皮细胞具有高血管生成潜力，可能作为血管内皮干细胞存在。
