Buster J E, Bustillo M, Rodi I A, Cohen S W, Hamilton M, Simon J A, Thorneycroft I H, Marshall J R
Am J Obstet Gynecol. 1985 Sep 15;153(2):211-7. doi: 10.1016/0002-9378(85)90116-4.
Using uterine lavage performed 5 days after the luteinizing hormone peak, we collected 25 uterine ova from five fertile donors who had had a single, periovular artificial insemination. After examination, all recovered ova were transferred to recipient uteri and resulted in three intrauterine and one tubal pregnancy. Morphologic development ranged from degenerating single-cell ova to mature blastocysts. Ages of the ova at recovery ranged from 93.5 to 130.0 hours postovulation. Mean age of the five blastocysts, 109.1 hours, was not significantly different from the mean age of the 20 less mature ova, 108.1 hours. Neither the mean interval from insemination to recovery for blastocysts nor the mean interval from insemination to ovulation for blastocysts was significantly different from the mean intervals of the other ova. The five blastocysts resulted in intrauterine pregnancies in three recipients. There were no intrauterine pregnancies from the other 20 transfers (p less than 0.004). A transferred 12-cell ovum with degenerating blastomeres was associated with tubal pregnancy in the recipient. The large variability in the state of intrauterine ova observed in the relatively fixed postovulatory interval of this study appears to be due to differences in viability and maturation, not differences in ovum age. Maturational state of the ova at the time of transfer appears to be a significant determinant of the likelihood of ensuring pregnancy.
在促黄体生成素峰值出现5天后进行子宫灌洗，我们从5名有过单次围排卵期人工授精的可育供体中收集了25个子宫内卵子。检查后，将所有回收的卵子移植到受体子宫内，结果有3例发生宫内妊娠，1例发生输卵管妊娠。形态学发育范围从退化的单细胞卵子到成熟的囊胚。回收时卵子的年龄在排卵后93.5至130.0小时之间。5个囊胚的平均年龄为109.1小时，与20个不太成熟卵子的平均年龄108.1小时无显著差异。囊胚从授精到回收的平均间隔以及囊胚从授精到排卵的平均间隔与其他卵子的平均间隔均无显著差异。5个囊胚在3名受体中导致了宫内妊娠。其他20次移植未发生宫内妊娠（p<0.004）。一个带有退化卵裂球的12细胞卵子移植后导致受体发生输卵管妊娠。在本研究相对固定的排卵后间隔内观察到的子宫内卵子状态的巨大差异似乎是由于活力和成熟度的差异，而不是卵子年龄的差异。移植时卵子的成熟状态似乎是确保妊娠可能性的一个重要决定因素。
