Anderson P J
Anal Biochem. 1985 Jul;148(1):105-10. doi: 10.1016/0003-2697(85)90634-7.
Conditions for the electroelution of protein from polyacrylamide gels to nitrocellulose and its subsequent recovery have been examined. A procedure is described whereby soluble material suitable for further analysis can be obtained. Nitrocellulose-bound protein is dissolved in acetone. It can be precipitated from solution in the presence of a carrier such as Polybrene in a form that can subsequently be solubilized in good yield.
研究了从聚丙烯酰胺凝胶中电洗脱蛋白质至硝酸纤维素膜并随后回收的条件。描述了一种方法，通过该方法可获得适合进一步分析的可溶性材料。与硝酸纤维素膜结合的蛋白质溶解于丙酮中。在诸如聚凝胺之类的载体存在下，它可以从溶液中沉淀出来，形成随后可高收率溶解的形式。
