Kocatepe Melissa, Ertan Kubra, Sahin Serpil, Sumnu Gulum
Department of Food Engineering, Middle East Technical University, 06800 Ankara, Turkiye.
Department of Food Engineering, Faculty of Engineering and Architecture, Burdur Mehmet Akif Ersoy University, Istiklal Campus, 15030 Burdur, Turkiye.
Int J Biol Macromol. 2025 Jun;312:144178. doi: 10.1016/j.ijbiomac.2025.144178. Epub 2025 May 14.
In this study, faba bean (Vicia faba L.) protein concentrate-based biodegradable films were developed using the casting method at two pH levels (6 and 8) and with different microbial transglutaminase (MTGase) concentrations (0, 5 U, and 10 U). Films prepared at pH 8 exhibited improved mechanical properties due to the higher solubility of proteins. Therefore, subsequent experiments were conducted with the films prepared at this pH. The films were evaluated for their barrier properties, color, antioxidant properties, thermal stability, and degree of cross-linking. The incorporation of 5 U MTGase led to random cross-link formation within the film matrix, which negatively impacted the mechanical and barrier properties. In contrast, 10 U MTGase enhanced the physical properties of the films and resulted in a higher degree of cross-linking. Thyme oil (Thymus vulgaris) was incorporated into the film-forming solutions to enhance their antioxidant and antimicrobial properties. While 1 % thyme oil enhanced the active properties of the films, it reduced their mechanical strength, barrier performance, and degree of cross-linking. Despite these challenges, incorporating 1 % thyme oil in films crosslinked with 10 U MTGase is recommended for developing active packaging films with high antioxidant capacity and antimicrobial effectiveness.
在本研究中，采用流延法在两个pH水平（6和8）以及不同微生物转谷氨酰胺酶（MTGase）浓度（0、5 U和10 U）下制备了基于蚕豆（Vicia faba L.）浓缩蛋白的可生物降解薄膜。在pH 8条件下制备的薄膜由于蛋白质具有更高的溶解度而表现出改善的机械性能。因此，随后对在此pH条件下制备的薄膜进行了实验。对薄膜的阻隔性能、颜色、抗氧化性能、热稳定性和交联度进行了评估。加入5 U MTGase会导致在薄膜基质内形成无规交联，这对机械性能和阻隔性能产生负面影响。相比之下，10 U MTGase增强了薄膜的物理性能并导致更高的交联度。将百里香油（Thymus vulgaris）加入到成膜溶液中以增强其抗氧化和抗菌性能。虽然1%的百里香油增强了薄膜的活性性能，但它降低了薄膜的机械强度、阻隔性能和交联度。尽管存在这些挑战，但对于开发具有高抗氧化能力和抗菌效果的活性包装薄膜，建议在与10 U MTGase交联的薄膜中加入1%的百里香油。
